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Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2 '-O-methyltransferase | |
2015-08-03 | |
发表期刊 | RNA BIOLOGY |
ISSN | 1547-6286 |
卷号 | 12期号:8页码:900-911 |
发表状态 | 已发表 |
DOI | 10.1080/15476286.2015.1050576 |
摘要 | Post-transcriptional modifications bring chemical diversity to tRNAs, especially at positions 34 and 37 of the anticodon stem-loop (ASL). TrmL is the prokaryotic methyltransferase that catalyzes the transfer of the methyl group from S-adenosyl-L-methionine to the wobble base of tRNA(CAA)(Leu) and tRNA(UAA)(Leu) isoacceptors. This Cm34/Um34 modification affects codon-anticodon interactions and is essential for translational fidelity. TrmL-catalyzed 2-O-methylation requires its homodimerization; however, understanding of the tRNA recognition mechanism by TrmL remains elusive. In the current study, by measuring tRNA methylation by TrmL and performing kinetic analysis of tRNA mutants, we found that TrmL exhibits a fine-tuned tRNA substrate recognition mechanism. Anticodon stem-loop minihelices with an extension of 2 base pairs are the minimal substrate for EcTrmL methylation. A35 is a key residue for TrmL recognition, while A36-A37-A38 are important either via direct interaction with TrmL or due to the necessity for prior isopentenylation (i(6)) at A37. In addition, TrmL only methylates pyrimidines but not purine residues at the wobble position, and the 2-O-methylation relies on prior N-6-isopentenyladenosine modification at position 37. |
关键词 | recognition determinants TrmL tRNA modification wobble base 2-O-methyltransferase |
收录类别 | SCI |
资助项目 | National Key Basic Research Foundation of China[2012CB911000] |
WOS研究方向 | Biochemistry & Molecular Biology |
WOS类目 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000359659700013 |
出版者 | TAYLOR & FRANCIS INC |
WOS关键词 | POSTTRANSCRIPTIONAL MODIFICATIONS ; STRUCTURAL REQUIREMENTS ; SALMONELLA-TYPHIMURIUM ; METHYLTRANSFERASE ; DOMAIN ; BIOSYNTHESIS ; SPECIFICITY ; GENES ; TRMD ; 1-METHYLGUANOSINE |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2158 |
专题 | 生命科学与技术学院_特聘教授组_王恩多组 生命科学与技术学院_PI研究组_刘如娟组 |
通讯作者 | Liu, Ru-Juan |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Zhou, Mi,Long, Tao,Fang, Zhi-Peng,et al. Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2 '-O-methyltransferase[J]. RNA BIOLOGY,2015,12(8):900-911. |
APA | Zhou, Mi,Long, Tao,Fang, Zhi-Peng,Zhou, Xiao-Long,Liu, Ru-Juan,&Wang, En-Duo.(2015).Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2 '-O-methyltransferase.RNA BIOLOGY,12(8),900-911. |
MLA | Zhou, Mi,et al."Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2 '-O-methyltransferase".RNA BIOLOGY 12.8(2015):900-911. |
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