Acidic phospholipids govern the enhanced activation of IgG-B cell receptor

doi:10.1038/ncomms9552

	Acidic phospholipids govern the enhanced activation of IgG-B cell receptor
	Chen, Xiangjun 1; Pan, Weiling 2; Sui, Yinqiang 1; Li, Hua 2; Shi, Xiaoshan 2; Guo, Xingdong 2; Qi, Hai 3; Xu, Chenqi2,4 ; Liu, Wanli 1
	2015-10
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	6
发表状态	已发表
DOI	10.1038/ncomms9552
摘要	B cells that express the isotype-switched IgG-B cell receptor (IgG-BCR) are one of the driving forces for antibody memory. To allow for a rapid memory IgG antibody response, IgG-BCR evolved into a highly effective signalling machine. Here, we report that the positively charged cytoplasmic domain of mIgG (mIgG-tail) specifically interacts with negatively charged acidic phospholipids. The key immunoglobulin tail tyrosine (ITT) in mIgG-tail is thus sequestered in the membrane hydrophobic core in quiescent B cells. Pre-disruption of such interaction leads to excessive recruitment of BCRs and inflated BCR signalling upon antigen stimulation, resulting in hyperproliferation of primary B cells. Physiologically, membrane-sequestered mIgG-tail can be released by antigen engagement or Ca2+ mobilization in the initiation of B cell activation. Our studies suggest a novel regulatory mechanism for how dynamic association of mIgG-tail with acidic phospholipids governs the enhanced activation of IgG-BCR.
收录类别	SCI
语种	英语
资助项目	Tsinghua University Initiative Scientific Research Program[20131089279]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000364930800037
出版者	NATURE PUBLISHING GROUP
WOS关键词	PROTEIN-LIPID INTERACTION ; ANTIGEN RECEPTOR ; CYTOPLASMIC TAIL ; MEMBRANE-BINDING ; NMR DATA ; MEMORY ; MOTIF ; PHOSPHORYLATION ; DOMAINS ; BCRS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2122
专题	生命科学与技术学院_特聘教授组_许琛琦组
通讯作者	Xu, Chenqi
作者单位	1.Tsinghua Univ, Sch Life Sci, Collaborat Innovat Ctr Diag & Treatment Infect Di, MOE Key Lab Prot Sci, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai,State Key Lab Mol Biol, Shanghai 200031, Peoples R China 3.Tsinghua Univ, Sch Med, Lab Dynam Immunobiol, Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Chen, Xiangjun,Pan, Weiling,Sui, Yinqiang,et al. Acidic phospholipids govern the enhanced activation of IgG-B cell receptor[J]. NATURE COMMUNICATIONS,2015,6.
APA	Chen, Xiangjun.,Pan, Weiling.,Sui, Yinqiang.,Li, Hua.,Shi, Xiaoshan.,...&Liu, Wanli.(2015).Acidic phospholipids govern the enhanced activation of IgG-B cell receptor.NATURE COMMUNICATIONS,6.
MLA	Chen, Xiangjun,et al."Acidic phospholipids govern the enhanced activation of IgG-B cell receptor".NATURE COMMUNICATIONS 6(2015).