Isothermal Amplification of Nucleic Acids

doi:10.1021/acs.chemrev.5b00428

	Isothermal Amplification of Nucleic Acids
	Zhao, Yongxi 1; Chen, Feng 1; Li, Qian 2,3; Wang, Lihua 2,3; Fan, Chunhai2,3,4
	2015-11-25
发表期刊	CHEMICAL REVIEWS
ISSN	0009-2665
卷号	115 期号:22 页码:12491-12545
发表状态	已发表
DOI	10.1021/acs.chemrev.5b00428
摘要	Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR). These isothermal amplification methods have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. The applications of these techniques for in situ or intracellular bioimaging and sequencing have been amply demonstrated. Amplicons produced by isothermal amplification methods have also been utilized to construct versatile nucleic acid nanomaterials for promising applications in biomedicine, bioimaging, and biosensing. The integration of isothermal amplification into microsystems or portable devices improves nucleic acid-based on-site assays and confers high sensitivity. Single-cell and single-molecule analyses have also been implemented based on integrated microfluidic systems. In this review, we provide a comprehensive overview of the isothermal amplification of nucleic acids encompassing work published in the past two decades. First, different isothermal amplification techniques are classified into three types based on reaction kinetics. Then, we summarize the applications of isothermal amplification in bioanalysis, diagnostics, nanotechnology, materials science, and device integration. Finally, several challenges and perspectives in the field are discussed.
收录类别	SCI ; EI
语种	英语
资助项目	Natural Science Foundation of Shaanxi Province[2013JQ2017]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000366005400009
出版者	AMER CHEMICAL SOC
EI入藏号	20154901637989
EI主题词	Biomolecules ; Isotherms ; Molecules ; Nucleic acid sequences ; Nucleic acids ; Reaction kinetics
EI分类号	Biochemistry:801.2 ; Chemical Reactions:802.2 ; Atomic and Molecular Physics:931.3
WOS关键词	ROLLING-CIRCLE-AMPLIFICATION ; HYBRIDIZATION CHAIN-REACTION ; STRAND-DISPLACEMENT AMPLIFICATION ; POLYNUCLEOTIDE KINASE-ACTIVITY ; HIGHLY SENSITIVE DETECTION ; ASSISTED SIGNAL AMPLIFICATION ; ULTRASENSITIVE ELECTROCHEMICAL DETECTION ; DNA METHYLTRANSFERASE ACTIVITY ; SINGLE-NUCLEOTIDE POLYMORPHISMS ; SEQUENCE-BASED AMPLIFICATION
原始文献类型	Review
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/2088
专题	生命科学与技术学院_特聘教授组_樊春海组
通讯作者	Zhao, Yongxi
作者单位	1.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Educ Minist, Key Lab Biomed Informat Engn, Xianning West Rd, Xian 710049, Shaanxi, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Laboraotory Interfacial Phys & Technol, Div Phys Biol,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Laboraotory Interfacial Phys & Technol, Bioimaging Ctr,Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yongxi,Chen, Feng,Li, Qian,et al. Isothermal Amplification of Nucleic Acids[J]. CHEMICAL REVIEWS,2015,115(22):12491-12545.
APA	Zhao, Yongxi,Chen, Feng,Li, Qian,Wang, Lihua,&Fan, Chunhai.(2015).Isothermal Amplification of Nucleic Acids.CHEMICAL REVIEWS,115(22),12491-12545.
MLA	Zhao, Yongxi,et al."Isothermal Amplification of Nucleic Acids".CHEMICAL REVIEWS 115.22(2015):12491-12545.