Distinct Proteome Remodeling of Industrial Saccharomyces cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock

doi:10.1021/acs.jproteome.7b00842

	Distinct Proteome Remodeling of Industrial Saccharomyces cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock
	Xiao, Weidi 1,3; Duan, Xiaoxiao 3,4; Lin, Yuping 4; Cao, Qichen 4; Li, Shanshan1 ; Guo, Yufeng 4; Gan, Yuman 4; Qi, Xianni 4; Zhou, Yue 5; Guo, Lihai 6; Qin, Peibin 6; Wang, Qinhong 4; Shui, Wenqing1,2
	2018-05
发表期刊	JOURNAL OF PROTEOME RESEARCH (IF:3.8[JCR-2023],4.1[5-Year])
ISSN	1535-3893
卷号	17 期号:5 页码:1812-1825
发表状态	已发表
DOI	10.1021/acs.jproteome.7b00842
摘要	To gain a deep understanding of yeast-cell response to heat stress, multiple laboratory strains have been intensively studied via genome-wide expression analysis for the mechanistic dissection of classical heat-shock response (HSR). However, robust industrial strains of Saccharomyces cerevisiae have hardly been explored in global analysis for elucidation of the mechanism of thermotolerant response (TR) during fermentation. Herein, we employed data-independent acquisition and sequential window acquisition of all theoretical mass spectra based proteomic workflows to characterize proteome remodeling of an industrial strain, ScY01, responding to prolonged thermal stress or transient heat shock. By comparing the proteomic signatures of ScY01 in TR versus HSR as well as the HSR of the industrial strain versus a laboratory strain, our study revealed disparate response mechanisms of ScY01 during thermotolerant growth or under heat shock. In addition, through proteomics data-mining for decoding transcription factor interaction networks followed by validation experiments, we uncovered the functions of two novel transcription factors, Mig1 and Srb2, in enhancing the thermotolerance of the industrial strain. This study has demonstrated that accurate and high-throughput quantitative proteomics not only provides new insights into the molecular basis for complex microbial phenotypes but also pinpoints upstream regulators that can be targeted for improving the desired traits of industrial microorganisms.
关键词	industrial yeast thermotolerance heat shock response transcription factor DIA SWATH
收录类别	SCI ; SCIE
语种	英语
资助项目	Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission[11ZCZDSY08400]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000431726700007
出版者	AMER CHEMICAL SOC
WOS关键词	DATA-INDEPENDENT ACQUISITION ; SWATH-MASS-SPECTROMETRY ; ENVIRONMENTAL-CHANGES ; ETHANOL-PRODUCTION ; YEAST-CELLS ; QUANTIFICATION ; THERMOTOLERANCE ; FERMENTATION ; MECHANISMS ; EXPRESSION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20871
专题	iHuman研究所_PI研究组_水雯箐组生命科学与技术学院
通讯作者	Wang, Qinhong; Shui, Wenqing
作者单位	1.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 5.Thermofisher Sci China, Demo Lab, Shanghai 200120, Peoples R China 6.AB SCIEX, 1 Bldg,24 Yard,Jiuxianqiao Mid Rd, Beijing 100015, Peoples R China
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所; 生命科学与技术学院
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Xiao, Weidi,Duan, Xiaoxiao,Lin, Yuping,et al. Distinct Proteome Remodeling of Industrial Saccharomyces cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(5):1812-1825.
APA	Xiao, Weidi.,Duan, Xiaoxiao.,Lin, Yuping.,Cao, Qichen.,Li, Shanshan.,...&Shui, Wenqing.(2018).Distinct Proteome Remodeling of Industrial Saccharomyces cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock.JOURNAL OF PROTEOME RESEARCH,17(5),1812-1825.
MLA	Xiao, Weidi,et al."Distinct Proteome Remodeling of Industrial Saccharomyces cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock".JOURNAL OF PROTEOME RESEARCH 17.5(2018):1812-1825.