Recently Evolved Tumor Suppressor Transcript TP73-AS1 Functions as Sponge of Human-Specific miR-941

doi:10.1093/molbev/msy022

KMS > 生命科学与技术学院 > 特聘教授组 > Philipp Khaitovich组

	Recently Evolved Tumor Suppressor Transcript TP73-AS1 Functions as Sponge of Human-Specific miR-941
	Hu, Haiyang 1,2; Liu, Jian-Mei 3,4; Hu, Zhenyu 3,4; Jiang, Xi 2; Yang, Xiaode 2,5; Li, Jiangxia 3,4; Zhang, Yao 3,4; Yu, Haijing 3,4; Khaitovich, Philipp6,7,8,9,10
	2018-05
发表期刊	MOLECULAR BIOLOGY AND EVOLUTION
ISSN	0737-4038
卷号	35 期号:5 页码:1063-1077
发表状态	已发表
DOI	10.1093/molbev/msy022
摘要	MicroRNA (miRNA) sponges are vital components of posttranscriptional gene regulation. Yet, only a limited number of miRNA sponges have been identified. Here, we show that the recently evolved noncoding tumor suppressor transcript, antisense RNA to TP73 gene (TP73-AS1), functions as a natural sponge of human-specific miRNA miR-941. We find unusually nine high-affinity miR-941 binding sites clustering within 1 kb region on TP73-AS1, which forms miR-941 sponge region. This sponge region displays increased sequence constraint only in humans, and its formation can be traced to the tandem expansion of a 71-nt-long sequence containing a single miR-941 binding site in old world monkeys. We further confirm TP73-AS1 functions as an efficient miR-941 sponge based on massive transcriptome data analyses, wound-healing assay, and Argonaute protein immunoprecipitation experiments conducted in cell lines. The expression of miR-941 and its sponge correlate inversely across multiple healthy and cancerous tissues, with miR-941 being highly expressed in tumors and preferentially repressing tumor suppressors. Thus, the TP73-AS1 and miR-941 duo represents an unusual case of the extremely rapid evolution of noncoding regulators controlling cell migration, proliferation, and tumorigenesis.
关键词	microRNA sponge young noncoding RNA co-evolution tumorigenesis
收录类别	SCI ; SCIE
语种	英语
资助项目	Science and Technology Key Project of Yunnan Province[2017FA009]
WOS研究方向	Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS记录号	WOS:000431889600003
出版者	OXFORD UNIV PRESS
WOS关键词	LONG NONCODING RNAS ; MICRORNA TARGET SITES ; NONHUMAN-PRIMATES ; MULTIPLE-MYELOMA ; GENE-EXPRESSION ; MESSENGER-RNAS ; GENOME BROWSER ; CIRCULAR RNAS ; CANCER ; EVOLUTION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20833
专题	生命科学与技术学院_特聘教授组_Philipp Khaitovich组
通讯作者	Yu, Haijing; Khaitovich, Philipp
作者单位	1.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing, Jiangsu, Peoples R China 2.CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai, Peoples R China 3.Yunnan Univ, Sch Life Sci, State Key Lab Nat Resource Conservat & Utilizat, Kunming, Yunnan, Peoples R China 4.Yunnan Univ, Sch Life Sci, Ctr Life Sci, Kunming, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.Skolkovo Inst Sci & Technol, Skolkovo, Russia 7.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China 8.CAS MPG Partner Inst Computat Biol, Comparat Biol Grp, Shanghai, Peoples R China 9.Max Planck Inst Evolutionary Anthropol, Leipzig, Germany 10.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Hu, Haiyang,Liu, Jian-Mei,Hu, Zhenyu,et al. Recently Evolved Tumor Suppressor Transcript TP73-AS1 Functions as Sponge of Human-Specific miR-941[J]. MOLECULAR BIOLOGY AND EVOLUTION,2018,35(5):1063-1077.
APA	Hu, Haiyang.,Liu, Jian-Mei.,Hu, Zhenyu.,Jiang, Xi.,Yang, Xiaode.,...&Khaitovich, Philipp.(2018).Recently Evolved Tumor Suppressor Transcript TP73-AS1 Functions as Sponge of Human-Specific miR-941.MOLECULAR BIOLOGY AND EVOLUTION,35(5),1063-1077.
MLA	Hu, Haiyang,et al."Recently Evolved Tumor Suppressor Transcript TP73-AS1 Functions as Sponge of Human-Specific miR-941".MOLECULAR BIOLOGY AND EVOLUTION 35.5(2018):1063-1077.