Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes
Liu, Qiaozhen1; Yang, Rui1; Huang, Xiuzhen1; Zhang, Hui1; He, Lingjuan1; Zhang, Libo1; Tian, Xueying1; Nie, Yu2,3; Hu, Shengshou2,3; Yan, Yan4; Zhang, Li5; Qiao, Zengyong6; Wang, Qing-Dong7; Lui, Kathy O.8; Zhou, Bin1,9,10
Source PublicationCELL RESEARCH

Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem cells (CSCs), are in clinical trials to investigate their ability to stimulate cardiac regeneration and repair. These studies were initially motivated by the purported cardiogenic activity of these cells. Recent lineage tracing studies using Kit promoter to drive expression of the inducible Cre recombinase showed that these CSCs had highly limited cardiogenic activity, inadequate to support efficient cardiac repair. Here we reassess the lineage tracing data by investigating the identity of cells immediately after Cre labeling. Our instant lineage tracing approach identifies Kit-expressing cardiomyocytes, which are labeled immediately after tamoxifen induction. In combination with long-term lineage tracing experiments, these data reveal that the large majority of long-term labeled cardiomyocytes are pre-existing Kit-expressing cardiomyocytes rather than cardiomyocytes formed de novo from CSCs. This study presents a new interpretation for the contribution of Kit(+) cells to cardiomyocytes and shows that Kit genetic lineage tracing over-estimates the cardiogenic activity of Kit(+) CSCs.

Keywordcardiac stem cell c-kit lineage tracing cardiomyocyte
Indexed BySCI
Funding ProjectResearch Grants Council of Hong Kong[24110515]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000367615200011
Original Document TypeArticle
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Cited Times:95[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorZhou, Bin
Affiliation1.Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab, Inst Nutr Sci,Grad Sch, Shanghai 200031, Peoples R China
2.Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, State Key Lab Cardiovasc Dis, Fuwai Hosp, Beijing 100037, Peoples R China
3.Peking Union Med Coll, Beijing 100037, Peoples R China
4.Fudan Univ, Zhongshan Hosp, Shanghai 200032, Peoples R China
5.Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Cardiol, Hangzhou 310003, Zhejiang, Peoples R China
6.Southern Med Univ, Affiliated Fengxian Hosp, Dept Cardiovasc Med, Shanghai 201499, Peoples R China
7.AstraZeneca, Cardiovasc & Metab Dis Innovat Med, S-43183 Molndal, Sweden
8.Chinese Univ Hong Kong, Prince Wales Hosp, Li Ka Shing Inst Hlth Sci, Dept Chem Pathol, Shatin 999077, Hong Kong, Peoples R China
9.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci,State Key Lab Neurosci, Shanghai 200031, Peoples R China
10.ShanghaiTech Univ, Shanghai 201210, Peoples R China
Corresponding Author AffilicationShanghaiTech University
Recommended Citation
GB/T 7714
Liu, Qiaozhen,Yang, Rui,Huang, Xiuzhen,et al. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes[J]. CELL RESEARCH,2016,26(1):119-130.
APA Liu, Qiaozhen.,Yang, Rui.,Huang, Xiuzhen.,Zhang, Hui.,He, Lingjuan.,...&Zhou, Bin.(2016).Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.CELL RESEARCH,26(1),119-130.
MLA Liu, Qiaozhen,et al."Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes".CELL RESEARCH 26.1(2016):119-130.
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