A metagenomic approach to dissect the genetic composition of enterotypes in Han Chinese and two Muslim groups

doi:10.1016/j.syapm.2017.09.006

	A metagenomic approach to dissect the genetic composition of enterotypes in Han Chinese and two Muslim groups
	Li, Jing 1,2; Fu, Ruiqing 1,3; Yang, Yajun 4,5; Horz, Hans-Peter 6; Guan, Yaqun 7; Lu, Yan 1; Lou, Haiyi 1; Tian, Lei 1,3; Zheng, Shijie 1; Liu, Hongjiao 1,8; Shi, Meng 1,3; Tang, Kun 1; Wang, Sijia 1; Xu, Shuhua1,3,9,10
	2018-01
发表期刊	SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN	0723-2020
卷号	41 期号:1 页码:1-12
发表状态	已发表
DOI	10.1016/j.syapm.2017.09.006
摘要	Distinct enterotypes have been observed in the human gut but little is known about the genetic basis of the microbiome. Moreover, it is not clear how many genetic differences exist between enterotypes within or between populations. In this study, both the 16S rRNA gene and the metagenomes of the gut microbiota were sequenced from 48 Han Chinese, 48 Kazaks, and 96 Uyghurs, and taxonomies were assigned after de novo assembly. Single nucleotide polymorphisms were also identified by referring to data from the Human Microbiome Project. Systematic analysis of the gut communities in terms of their abundance and genetic composition was also performed, together with a genome-wide association study of the host genomes. The gut microbiota of 192 subjects was clearly classified into two enterotypes (Bacteroides and Prevotella). Interestingly, both enterotypes showed a clear genetic differentiation in terms of their functional catalogue of genes, especially for genes involved in amino acid and carbohydrate metabolism. In addition, several differentiated genera and genes were found among the three populations. Notably, one human variant (rs878394) was identified that showed significant association with the abundance of Prevotella, which is linked to LYPIAL1, a gene associated with body fat distribution, the waist-hip ratio and insulin sensitivity. Taken together, considerable differentiation was observed in gut microbes between enterotypes and among populations that was reflected in both the taxonomic composition and the genetic makeup of their functional genes, which could have been influenced by a variety of factors, such as diet and host genetic variation. (C) 2017 Elsevier GmbH. All rights reserved.
关键词	Metagenome Next-generation sequencing 16S rRNA Enterotype Genome-wide association study
收录类别	SCI ; SCIE
语种	英语
资助项目	STCSM grant[16YF1413900]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000429912800001
出版者	ELSEVIER GMBH
WOS关键词	GUT MICROBIOTA ; ASSOCIATION ; COMMUNITY ; ALIGNMENT ; CHILDREN ; TOOL
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/20211
专题	生命科学与技术学院_特聘教授组_徐书华组
通讯作者	Li, Jing; Xu, Shuhua
作者单位	1.Chinese Acad Sci, Max Planck Independent Res Grp Populat Genom, CAS MPG Partner Inst Computat Biol PICB, Key Lab Computat Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 2.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Jiangsu, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China 5.Fudan Univ, Sch Life Sci, Minist Educ MOE, Key Lab Contemporary Anthropol, Shanghai 200433, Peoples R China 6.RWTH Aachen Univ Hosp, Inst Med Microbiol, D-52074 Aachen, Germany 7.Xinjiang Med Univ, Dept Biochem, Preclin Med Coll, Urumqi 830011, Peoples R China 8.Johns Hopkins Univ, Dept Appl Math & Stat, Baltimore, MD 21218 USA 9.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 10.Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Jing,Fu, Ruiqing,Yang, Yajun,et al. A metagenomic approach to dissect the genetic composition of enterotypes in Han Chinese and two Muslim groups[J]. SYSTEMATIC AND APPLIED MICROBIOLOGY,2018,41(1):1-12.
APA	Li, Jing.,Fu, Ruiqing.,Yang, Yajun.,Horz, Hans-Peter.,Guan, Yaqun.,...&Xu, Shuhua.(2018).A metagenomic approach to dissect the genetic composition of enterotypes in Han Chinese and two Muslim groups.SYSTEMATIC AND APPLIED MICROBIOLOGY,41(1),1-12.
MLA	Li, Jing,et al."A metagenomic approach to dissect the genetic composition of enterotypes in Han Chinese and two Muslim groups".SYSTEMATIC AND APPLIED MICROBIOLOGY 41.1(2018):1-12.