Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes

doi:10.1038/cr.2016.6

	Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes
	Shi, Xiao-Lei 1; Gao, Yimeng 2; Yan, Yupeng 2; Ma, Hucheng 1; Sun, Lulu 2; Huang, Pengyu2,3 ; Ni, Xuan 4; Zhang, Ludi 2; Zhao, Xin 1; Ren, Haozhen 1; Hu, Dan 5; Zhou, Yan 5; Tian, Feng 6; Ji, Yuan 6; Cheng, Xin 2; Pan, Guoyu 4; Ding, Yi-Tao 1; Hui, Lijian2,3
	2016-02
发表期刊	CELL RESEARCH
ISSN	1001-0602
卷号	26 期号:2 页码:206-216
发表状态	已发表
DOI	10.1038/cr.2016.6
摘要	Acute liver failure (ALF) is a life-threatening illness. The extracorporeal cell-based bioartificial liver (BAL) system could bridge liver transplantation and facilitate liver regeneration for ALF patients by providing metabolic detoxification and synthetic functions. Previous BAL systems, based on hepatoma cells and non-human hepatocytes, achieved limited clinical advances, largely due to poor hepatic functions, cumbersome preparation or safety concerns of these cells. We previously generated human functional hepatocytes by lineage conversion (hiHeps). Here, by improving functional maturity of hiHeps and producing hiHeps at clinical scales (3 billion cells), we developed a hiHep-based BAL system (hiHep-BAL). In a porcine ALF model, hiHep-BAL treatment restored liver functions, corrected blood levels of ammonia and bilirubin, and prolonged survival. Importantly, human albumin and alpha-1-antitrypsin were detectable in hiHep-BAL-treated ALF pigs. Moreover, hiHep-BAL treatment led to attenuated liver damage, resolved inflammation and enhanced liver regeneration. Our findings indicate a promising clinical application of the hiHep-BAL system.
关键词	acute liver failure functional hepatocytes bioartificial liver
收录类别	SCI
语种	英语
资助项目	STCSM[14YF1406900]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000372062300008
出版者	NATURE PUBLISHING GROUP
WOS关键词	IN-VITRO EVALUATION ; SUPPORT-SYSTEM ; CONTROLLED-TRIAL ; ASSIST DEVICE ; CELLS ; TRANSPLANTATION ; PRESERVATION ; REVERSAL ; SAFETY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1929
专题	生命科学与技术学院_特聘教授组_惠利健组生命科学与技术学院_PI研究组_黄鹏羽组
通讯作者	Pan, Guoyu; Ding, Yi-Tao; Hui, Lijian
作者单位	1.Nanjing Univ, Sch Med, Dept Hepatobiliary Surg, Affiliated Drum Tower Hosp, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai 200031, Peoples R China 5.E China Univ Sci & Technol, Sch Bioengn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 6.Fudan Univ, Dept Pathol, Zhongshan Hosp, Shanghai 200433, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Shi, Xiao-Lei,Gao, Yimeng,Yan, Yupeng,et al. Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes[J]. CELL RESEARCH,2016,26(2):206-216.
APA	Shi, Xiao-Lei.,Gao, Yimeng.,Yan, Yupeng.,Ma, Hucheng.,Sun, Lulu.,...&Hui, Lijian.(2016).Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes.CELL RESEARCH,26(2),206-216.
MLA	Shi, Xiao-Lei,et al."Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes".CELL RESEARCH 26.2(2016):206-216.