Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins

doi:10.1038/ng.3486

	Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins
	Peng, Jamy C.1,2,6; Valouev, Anton 3; Liu, Na 1,2; Lin, Haifan1,2,4,5
	2016-03
发表期刊	NATURE GENETICS
ISSN	1061-4036
卷号	48 期号:3 页码:283-291
发表状态	已发表
DOI	10.1038/ng.3486
摘要	The Drosophila melanogaster Piwi protein regulates both niche and intrinsic mechanisms to maintain germline stem cells, but its underlying mechanism remains unclear. Here we report that Piwi interacts with Polycomb group complexes PRC1 and PRC2 in niche and germline cells to regulate ovarian germline stem cells and oogenesis. Piwi physically interacts with the PRC2 subunits Su(z)12 and Esc in the ovary and in vitro. Chromatin coimmunoprecipitation of Piwi, the PRC2 enzymatic subunit E(z), histone H3 trimethylated at lysine 27 (H3K27me3) and RNA polymerase II in wild-type and piwi mutant ovaries demonstrates that Piwi binds a conserved DNA motif at similar to 72 genomic sites and inhibits PRC2 binding to many non-Piwi-binding genomic targets and H3K27 trimethylation. Moreover, Piwi influences RNA polymerase II activities in Drosophila ovaries, likely via inhibiting PRC2. We hypothesize that Piwi negatively regulates PRC2 binding by sequestering PRC2 in the nucleoplasm, thus reducing PRC2 binding to many targets and influencing transcription during oogenesis.
收录类别	SCI
语种	英语
资助项目	US National Institutes of Health[1200-HD071011]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000370902600012
出版者	NATURE PUBLISHING GROUP
WOS关键词	HISTONE METHYLTRANSFERASE ACTIVITY ; CAENORHABDITIS-ELEGANS ; PIRNA PATHWAY ; TUDOR-DOMAIN ; PC-G ; MECHANISM ; INTERACTS ; GENOME ; DIVISION ; BINDING
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1918
专题	生命科学与技术学院免疫化学研究所_特聘教授组_干细胞生物学实验室
通讯作者	Peng, Jamy C.; Lin, Haifan
作者单位	1.Yale Univ, Yale Stem Cell Ctr, New Haven, CT USA 2.Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA 3.Univ So Calif, Keck Sch Med, Dept Prevent Med, Div Bioinformat, Los Angeles, CA 90033 USA 4.Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.St Jude Childrens Res Hosp, Dept Dev Neurobiol, Div Dev Biol, 332 N Lauderdale St, Memphis, TN 38105 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Peng, Jamy C.,Valouev, Anton,Liu, Na,et al. Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins[J]. NATURE GENETICS,2016,48(3):283-291.
APA	Peng, Jamy C.,Valouev, Anton,Liu, Na,&Lin, Haifan.(2016).Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins.NATURE GENETICS,48(3),283-291.
MLA	Peng, Jamy C.,et al."Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins".NATURE GENETICS 48.3(2016):283-291.