Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins
Peng, Jamy C.1,2,6; Valouev, Anton3; Liu, Na1,2; Lin, Haifan1,2,4,5
2016-03
Source PublicationNATURE GENETICS
ISSN1061-4036
Volume48Issue:3Pages:283-291
Status已发表
DOI10.1038/ng.3486
AbstractThe Drosophila melanogaster Piwi protein regulates both niche and intrinsic mechanisms to maintain germline stem cells, but its underlying mechanism remains unclear. Here we report that Piwi interacts with Polycomb group complexes PRC1 and PRC2 in niche and germline cells to regulate ovarian germline stem cells and oogenesis. Piwi physically interacts with the PRC2 subunits Su(z)12 and Esc in the ovary and in vitro. Chromatin coimmunoprecipitation of Piwi, the PRC2 enzymatic subunit E(z), histone H3 trimethylated at lysine 27 (H3K27me3) and RNA polymerase II in wild-type and piwi mutant ovaries demonstrates that Piwi binds a conserved DNA motif at similar to 72 genomic sites and inhibits PRC2 binding to many non-Piwi-binding genomic targets and H3K27 trimethylation. Moreover, Piwi influences RNA polymerase II activities in Drosophila ovaries, likely via inhibiting PRC2. We hypothesize that Piwi negatively regulates PRC2 binding by sequestering PRC2 in the nucleoplasm, thus reducing PRC2 binding to many targets and influencing transcription during oogenesis.
Indexed BySCI
Language英语
Funding ProjectUS National Institutes of Health[1200-HD071011]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000370902600012
PublisherNATURE PUBLISHING GROUP
WOS KeywordHISTONE METHYLTRANSFERASE ACTIVITY ; CAENORHABDITIS-ELEGANS ; PIRNA PATHWAY ; TUDOR-DOMAIN ; PC-G ; MECHANISM ; INTERACTS ; GENOME ; DIVISION ; BINDING
Original Document TypeArticle
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1918
Collection生命科学与技术学院
免疫化学研究所_特聘教授组_干细胞生物学实验室
Corresponding AuthorPeng, Jamy C.; Lin, Haifan
Affiliation1.Yale Univ, Yale Stem Cell Ctr, New Haven, CT USA
2.Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA
3.Univ So Calif, Keck Sch Med, Dept Prevent Med, Div Bioinformat, Los Angeles, CA 90033 USA
4.Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
6.St Jude Childrens Res Hosp, Dept Dev Neurobiol, Div Dev Biol, 332 N Lauderdale St, Memphis, TN 38105 USA
Corresponding Author AffilicationShanghai Institute for Advanced Immunochemical Studies;  School of Life Science and Technology
Recommended Citation
GB/T 7714
Peng, Jamy C.,Valouev, Anton,Liu, Na,et al. Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins[J]. NATURE GENETICS,2016,48(3):283-291.
APA Peng, Jamy C.,Valouev, Anton,Liu, Na,&Lin, Haifan.(2016).Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins.NATURE GENETICS,48(3),283-291.
MLA Peng, Jamy C.,et al."Piwi maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins".NATURE GENETICS 48.3(2016):283-291.
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