Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9

doi:10.1016/j.jgg.2016.02.003

	Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9
	Bai, Meizhu1,2,3 ; Liang, Dan 2,3; Wang, Yinghua 2,3; Li, Qing 2,3; Wu, Yuxuan 2,3; Li, Jinsong1,2,3
	2016-05-20
发表期刊	JOURNAL OF GENETICS AND GENOMICS (IF:6.6[JCR-2023],5.8[5-Year])
ISSN	1673-8527
卷号	43 期号:5 页码:289-296
发表状态	已发表
DOI	10.1016/j.jgg.2016.02.003
摘要	Tissue-specific knockout technology enables the analysis of the gene function in specific tissues in adult mammals. However, conventional strategy for producing tissue-specific knockout mice is a time- and labor-consuming process, restricting rapid study of the gene function in vivo. CRISPR-Cas9 system from bacteria is a simple and efficient gene-editing technique, which has enabled rapid generation of gene knockout lines in mouse by direct injection of CRISPR-Cas9 into zygotes. Here, we demonstrate CRISPR-Cas9-mediated spermatogenic cell-specific disruption of Scp3 gene in testes in one step. We first generated transgenic mice by pronuclear injection of a plasmid containing Hspa2 promoter driving Cas9 expression and showed Cas9 specific expression in spermatogenic cells. We then produced transgenic mice carrying Hspa2 promoter driven Cas9 and constitutive expressed sgRNA targeting Scp3 gene. Male founders were infertile due to developmental arrest of spermatogenic cells while female founders could produce progeny normally. Consistently, male progeny from female founders were infertile and females could transmit the transgenes to the next generation. Our study establishes a CRISPR-Cas9-based one-step strategy to analyze the gene function in adult tissues by a temporal-spatial pattern.
关键词	CRISPR-Cas9 Tissue-specific knockout Spermatogenesis Mouse
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Sciences[XDA01010403]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000376763300008
出版者	SCIENCE PRESS
WOS关键词	ONE-STEP GENERATION ; TRANSGENIC MICE ; GERMLINE TRANSMISSION ; CRE RECOMBINASE ; C. ELEGANS ; MOUSE ; DISRUPTION ; EXPRESSION ; PROTEIN ; EMBRYOS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1832
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_李劲松组生命科学与技术学院_博士生
通讯作者	Wu, Yuxuan; Li, Jinsong
作者单位	1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Grp Epigenet Reprogrammi, CAS Ctr Excellence Mol Cell Sci,State Key Lab Cel, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Bai, Meizhu,Liang, Dan,Wang, Yinghua,et al. Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9[J]. JOURNAL OF GENETICS AND GENOMICS,2016,43(5):289-296.
APA	Bai, Meizhu,Liang, Dan,Wang, Yinghua,Li, Qing,Wu, Yuxuan,&Li, Jinsong.(2016).Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9.JOURNAL OF GENETICS AND GENOMICS,43(5),289-296.
MLA	Bai, Meizhu,et al."Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9".JOURNAL OF GENETICS AND GENOMICS 43.5(2016):289-296.