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| Insights into the inhibitory mechanisms of NADH on the alpha gamma heterodimer of human NAD-dependent isocitrate dehydrogenase | |
| 2018-02-16 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 8 |
| Status | 已发表 |
| DOI | 10.1038/s41598-018-21584-7 |
| Abstract | Human NAD-dependent isocitrate dehydrogenase (NAD-IDH) catalyzes the oxidative decarboxylation of isocitrate in the citric acid cycle. In the alpha(2)beta gamma heterotetramer of NAD-IDH, the gamma subunit plays the regulatory role and the beta subunit the structural role. Previous biochemical data have shown that mammalian NAD-IDHs can be inhibited by NADH; however, the molecular mechanism is unclear. In this work, we show that the alpha beta, alpha gamma and alpha(2)beta gamma enzymes of human NAD-IDH can be inhibited by NADH, and further determine the crystal structure of the alpha gamma heterodimer bound with an Mg2+ and an NADH at the active site and an NADH at the allosteric site, which resembles that of the inactive alpha(Mg)gamma heterodimer. The NADH at the active site occupies the binding site for NAD(+) and prevents the binding of the cofactor. The NADH at the allosteric site occupies the binding sites for ADP and citrate and blocks the binding of the activators. The biochemical data confirm that the NADH binding competes with the binding of NAD(+) and the binding of citrate and ADP, and the two effects together contribute to the NADH inhibition on the activity. These findings provide insights into the inhibitory mechanisms of the alpha gamma heterodimer by NADH. |
| Indexed By | SCI ; SCIE |
| Language | 英语 |
| Funding Project | Chinese Academy of Sciences[XDB08010302] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000425284900014 |
| Publisher | NATURE PUBLISHING GROUP |
| WOS Keyword | AMINO-ACID-SEQUENCES ; PIG-HEART ; MOLECULAR-CLONING ; BETA-SUBUNITS ; BOVINE HEART ; CITRATE ; ADP |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/18286 |
| Collection | 生命科学与技术学院_特聘教授组_丁建平组 |
| Corresponding Author | Ding, Jianping |
| Affiliation | 1.Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China 2.Chinese Acad Sci, Natl Ctr Prot Sci Shanghai, Inst Biochem & Cell Biol,Shanghai Sci Res Ctr, Shanghai Inst Biol Sci,State Key Lab Mol Biol,CAS, 320Yue Yang Rd, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Hua Xia Zhong Rd, Shanghai 201210, Peoples R China |
| Corresponding Author Affilication | School of Life Science and Technology |
| Recommended Citation GB/T 7714 | Liu, Yabing,Hu, Lejia,Ma, Tengfei,et al. Insights into the inhibitory mechanisms of NADH on the alpha gamma heterodimer of human NAD-dependent isocitrate dehydrogenase[J]. SCIENTIFIC REPORTS,2018,8. |
| APA | Liu, Yabing,Hu, Lejia,Ma, Tengfei,Yang, Jun,&Ding, Jianping.(2018).Insights into the inhibitory mechanisms of NADH on the alpha gamma heterodimer of human NAD-dependent isocitrate dehydrogenase.SCIENTIFIC REPORTS,8. |
| MLA | Liu, Yabing,et al."Insights into the inhibitory mechanisms of NADH on the alpha gamma heterodimer of human NAD-dependent isocitrate dehydrogenase".SCIENTIFIC REPORTS 8(2018). |
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