Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

doi:10.1085/jgp.201611587

	Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology
	Barthmes, Maria 1,2,3,4; Liao, Jun5,7 ; Jiang, Youxing 5,6; Brueggemann, Andrea 1; Wahl-Schott, Christian 2,3,4
	2016-06
发表期刊	JOURNAL OF GENERAL PHYSIOLOGY
ISSN	0022-1295
卷号	147 期号:6 页码:485-496
发表状态	已发表
DOI	10.1085/jgp.201611587
摘要	Sodium-calcium exchangers (NCXs) are membrane transporters that play an important role in Ca2+ homeostasis and Ca2+ signaling. The recent crystal structure of NCX_Mj, a member of the NCX family from the archaebacterium Methanococcus jannaschii, provided insight into the atomistic details of sodium-calcium exchange. Here, we extend these findings by providing detailed functional data on purified NCX_Mj using solid supported membrane (SSM)-based electrophysiology, a powerful but unexploited tool for functional studies of electrogenic transporter proteins. We show that NCX_Mj is highly selective for Na+, whereas Ca2+ can be replaced by Mg2+ and Sr2+ and that NCX_Mj can be inhibited by divalent ions, particularly Cd2+. By directly comparing the apparent affinities of Na+ and Ca2+ for NCX_Mj with those for human NCX1, we show excellent agreement, indicating a strong functional similarity between NCX_Mj and its eukaryotic isoforms. We also provide detailed instructions to facilitate the adaption of this method to other electrogenic transporter proteins. Our findings demonstrate that NCX_Mj can serve as a model for the NCX family and highlight several possible applications for SSM-based electrophysiology.
收录类别	SCI
语种	英语
资助项目	German Research Foundation[SFB/TRR 152 TP06] ; German Research Foundation[SFB 870 TP B05]
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000377714400004
出版者	ROCKEFELLER UNIV PRESS
WOS关键词	SODIUM-CALCIUM EXCHANGE ; SODIUM/CALCIUM EXCHANGER ; CHANNEL ; TRANSLOCATION ; CONTRACTION ; PROTEINS ; BILAYERS ; GUIDE ; CA2+
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1824
专题	生命科学与技术学院_PI研究组_廖军组
通讯作者	Wahl-Schott, Christian
作者单位	1.Nan Technol, D-80636 Munich, Germany 2.Univ Munich, Dept Pharmacol, Ctr Integrated Prot Sci CIPS M, Marchioninistr 15, D-81377 Munich, Germany 3.Univ Munich, Dept Pharmacol, Ctr Drug Res, Marchioninistr 15, D-81377 Munich, Germany 4.Partner Site Munich Heart Alliance, DZHK German Ctr Cardiovasc Res, D-81377 Munich, Germany 5.Univ Texas SW Med Ctr Dallas, Dept Physiol, Dallas, TX 75390 USA 6.Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA 7.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Barthmes, Maria,Liao, Jun,Jiang, Youxing,et al. Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology[J]. JOURNAL OF GENERAL PHYSIOLOGY,2016,147(6):485-496.
APA	Barthmes, Maria,Liao, Jun,Jiang, Youxing,Brueggemann, Andrea,&Wahl-Schott, Christian.(2016).Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology.JOURNAL OF GENERAL PHYSIOLOGY,147(6),485-496.
MLA	Barthmes, Maria,et al."Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology".JOURNAL OF GENERAL PHYSIOLOGY 147.6(2016):485-496.