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| Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology | |
| 2016-06 | |
| Source Publication | JOURNAL OF GENERAL PHYSIOLOGY
 |
| ISSN | 0022-1295 |
| Volume | 147Issue:6Pages:485-496 |
| Status | 已发表 |
| DOI | 10.1085/jgp.201611587 |
| Abstract | Sodium-calcium exchangers (NCXs) are membrane transporters that play an important role in Ca2+ homeostasis and Ca2+ signaling. The recent crystal structure of NCX_Mj, a member of the NCX family from the archaebacterium Methanococcus jannaschii, provided insight into the atomistic details of sodium-calcium exchange. Here, we extend these findings by providing detailed functional data on purified NCX_Mj using solid supported membrane (SSM)-based electrophysiology, a powerful but unexploited tool for functional studies of electrogenic transporter proteins. We show that NCX_Mj is highly selective for Na+, whereas Ca2+ can be replaced by Mg2+ and Sr2+ and that NCX_Mj can be inhibited by divalent ions, particularly Cd2+. By directly comparing the apparent affinities of Na+ and Ca2+ for NCX_Mj with those for human NCX1, we show excellent agreement, indicating a strong functional similarity between NCX_Mj and its eukaryotic isoforms. We also provide detailed instructions to facilitate the adaption of this method to other electrogenic transporter proteins. Our findings demonstrate that NCX_Mj can serve as a model for the NCX family and highlight several possible applications for SSM-based electrophysiology. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | German Research Foundation[SFB/TRR 152 TP06] ; German Research Foundation[SFB 870 TP B05] |
| WOS Research Area | Physiology |
| WOS Subject | Physiology |
| WOS ID | WOS:000377714400004 |
| Publisher | ROCKEFELLER UNIV PRESS |
| WOS Keyword | SODIUM-CALCIUM EXCHANGE ; SODIUM/CALCIUM EXCHANGER ; CHANNEL ; TRANSLOCATION ; CONTRACTION ; PROTEINS ; BILAYERS ; GUIDE ; CA2+ |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1824 |
| Collection | 生命科学与技术学院_PI研究组_廖军组 |
| Corresponding Author | Wahl-Schott, Christian |
| Affiliation | 1.Nan Technol, D-80636 Munich, Germany 2.Univ Munich, Dept Pharmacol, Ctr Integrated Prot Sci CIPS M, Marchioninistr 15, D-81377 Munich, Germany 3.Univ Munich, Dept Pharmacol, Ctr Drug Res, Marchioninistr 15, D-81377 Munich, Germany 4.Partner Site Munich Heart Alliance, DZHK German Ctr Cardiovasc Res, D-81377 Munich, Germany 5.Univ Texas SW Med Ctr Dallas, Dept Physiol, Dallas, TX 75390 USA 6.Univ Texas SW Med Ctr Dallas, Howard Hughes Med Inst, Dallas, TX 75390 USA 7.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
| Recommended Citation GB/T 7714 | Barthmes, Maria,Liao, Jun,Jiang, Youxing,et al. Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology[J]. JOURNAL OF GENERAL PHYSIOLOGY,2016,147(6):485-496. |
| APA | Barthmes, Maria,Liao, Jun,Jiang, Youxing,Brueggemann, Andrea,&Wahl-Schott, Christian.(2016).Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology.JOURNAL OF GENERAL PHYSIOLOGY,147(6),485-496. |
| MLA | Barthmes, Maria,et al."Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology".JOURNAL OF GENERAL PHYSIOLOGY 147.6(2016):485-496. |
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