Post-Translational Modifications of G Protein-Coupled Receptors Revealed by Proteomics and Structural Biology

doi:10.3389/fchem.2022.843502

	Post-Translational Modifications of G Protein-Coupled Receptors Revealed by Proteomics and Structural Biology
	Zhang, Bingjie1 ; Li, Shanshan1 ; Shui, Wenqing1,2
	2022-03-10
发表期刊	FRONTIERS IN CHEMISTRY (IF:3.8[JCR-2023],4.8[5-Year])
ISSN	2296-2646
卷号	10
发表状态	已发表
DOI	10.3389/fchem.2022.843502
摘要	G protein-coupled receptors (GPCRs) are a protein superfamily comprising >800 members that regulate numerous cellular and physiologic responses. GPCRs represent the largest class of therapeutic targets with implications in various diseases. Although advances in GPCR structural and pharmacological research have significantly improved our knowledge of GPCR signaling mechanisms, mapping diverse post-translational modifications (PTMs) of GPCR proteins and understanding their regulatory roles have received much less attention. Mass spectrometry-based proteomics has become the most popular technology for profiling protein PTMs in a systematic manner. Herein we provide an overview of PTM types, locations, crosstalk and dynamic regulation for different GPCRs that are characterized using proteomic and/or biochemical approaches. Our main focus is on glycosylation, phosphorylation, ubiquitination and palmitoylation that are known to modulate receptor folding, biosynthesis, trafficking, dimerization and signaling. Furthermore, we discuss the locations of specific PTM sites in the structure of a given GPCR and its signaling complex to highlight the importance of PTM regulation in the molecular basis of GPCRs, which may shed new light on structure-based drug discovery.
关键词	G protein couped receptors mass spectrometry-based proteomics Post-translational modification (PTM) phosphorylation signaling regulation
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Program on Key Basic Research Project of China[2018YFA0507004] ; National Natural Science Foundation of China[31971362,32171439]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000787162400001
出版者	FRONTIERS MEDIA SA
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/180879
专题	iHuman研究所_PI研究组_水雯箐组生命科学与技术学院
通讯作者	Shui, Wenqing
作者单位	1.ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
第一作者单位	iHuman研究所
通讯作者单位	iHuman研究所; 生命科学与技术学院
第一作者的第一单位	iHuman研究所
推荐引用方式 GB/T 7714	Zhang, Bingjie,Li, Shanshan,Shui, Wenqing. Post-Translational Modifications of G Protein-Coupled Receptors Revealed by Proteomics and Structural Biology[J]. FRONTIERS IN CHEMISTRY,2022,10.
APA	Zhang, Bingjie,Li, Shanshan,&Shui, Wenqing.(2022).Post-Translational Modifications of G Protein-Coupled Receptors Revealed by Proteomics and Structural Biology.FRONTIERS IN CHEMISTRY,10.
MLA	Zhang, Bingjie,et al."Post-Translational Modifications of G Protein-Coupled Receptors Revealed by Proteomics and Structural Biology".FRONTIERS IN CHEMISTRY 10(2022).