Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments

doi:10.1074/jbc.M116.720557

	Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments
	Lu, Jun-Xia1,2 ; Bayro, Marvin J.1; Tycko, Robert 1
	2016-06-17
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN	0021-9258
卷号	291 期号:25 页码:13098-13112
发表状态	已发表
DOI	10.1074/jbc.M116.720557
摘要	We present the results of solid state nuclear magnetic resonance (NMR) experiments on HIV-1 capsid protein (CA) assemblies with three different morphologies, namely wild-type CA (WT-CA) tubes with 35-60 nm diameters, planar sheets formed by the Arg(18)-Leu mutant (R18L-CA), and R18L-CA spheres with 20-100 nm diameters. The experiments are intended to elucidate molecular structural variations that underlie these variations in CA assembly morphology. We find that multidimensional solid state NMR spectra of N-15, C-13-labeled CA assemblies are remarkably similar for the three morphologies, with only small differences in N-15 and C-13 chemical shifts, no significant differences in NMR line widths, and few differences in the number of detectable NMR cross-peaks. Thus, the pronounced differences in morphology do not involve major differences in the conformations and identities of structurally ordered protein segments. Instead, morphological variations are attributable to variations in conformational distributions within disordered segments, which do not contribute to the solid state NMR spectra. Variations in solid state NMR signals from certain amino acid side chains are also observed, suggesting differences in the intermolecular dimerization interface between curved and planar CA lattices, as well as possible differences in intramolecular helix-helix packing.
收录类别	SCI ; EI
语种	英语
资助项目	NIDDK, National Institutes of Health[DK075032]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000379770500018
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
EI入藏号	20162502527510
EI主题词	Conformations ; Interface states ; Light polarization ; Morphology ; Nuclear magnetic resonance spectroscopy ; Proteins
EI分类号	Light/Optics:741.1 ; Physical Chemistry:801.4 ; Organic Compounds:804.1 ; Classical Physics; Quantum Theory; Relativity:931 ; High Energy Physics; Nuclear Physics; Plasma Physics:932 ; Materials Science:951
WOS关键词	HUMAN-IMMUNODEFICIENCY-VIRUS ; SOLID-STATE NMR ; NUCLEAR-MAGNETIC-RESONANCE ; ANGLE-SPINNING NMR ; CHEMICAL-SHIFTS ; GAG POLYPROTEIN ; TERMINAL DOMAIN ; DIMERIZATION DOMAIN ; CRYSTAL-STRUCTURE ; LATTICE FORMATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1801
专题	生命科学与技术学院_PI研究组_陆珺霞组
通讯作者	Tycko, Robert
作者单位	1.NIDKK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA 2.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
第一作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Lu, Jun-Xia,Bayro, Marvin J.,Tycko, Robert. Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(25):13098-13112.
APA	Lu, Jun-Xia,Bayro, Marvin J.,&Tycko, Robert.(2016).Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments.JOURNAL OF BIOLOGICAL CHEMISTRY,291(25),13098-13112.
MLA	Lu, Jun-Xia,et al."Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments".JOURNAL OF BIOLOGICAL CHEMISTRY 291.25(2016):13098-13112.