Disruption of FGF5 in Cashmere Goats Using CRISPR/Cas9 Results in More Secondary Hair Follicles and Longer Fibers

doi:10.1371/journal.pone.0164640

	Disruption of FGF5 in Cashmere Goats Using CRISPR/Cas9 Results in More Secondary Hair Follicles and Longer Fibers
	Wang, Xiaolong 1; Cai, Bei 1; Zhou, Jiankui3 ; Zhu, Haijing 4,5; Niu, Yiyuan 1; Ma, Baohua 6; Yu, Honghao 4,5; Lei, Anmin 6; Yan, Hailong 1,4,5; Shen, Qiaoyan 6; Shi, Lei 4,5; Zhao, Xiaoe 6; Hua, Jinlian 6; Huang, Xingxu2,3 ; Qu, Lei 4,5; Chen, Yulin 1
	2016-10-18
发表期刊	PLOS ONE (IF:2.9[JCR-2023],3.3[5-Year])
ISSN	1932-6203
卷号	11 期号:10
发表状态	已发表
DOI	10.1371/journal.pone.0164640
摘要	Precision genetic engineering accelerates the genetic improvement of livestock for agriculture and biomedicine. We have recently reported our success in producing gene-modified goats using the CRISPR/Cas9 system through microinjection of Cas9 mRNA and sgRNAs targeting the MSTN and FGF5 genes in goat embryos. By investigating the influence of gene modification on the phenotypes of Cas9-mediated goats, we herein demonstrate that the utility of this approach involving the disruption of FGF5 results in increased number of second hair follicles and enhanced fiber length in Cas9-mediated goats, suggesting more cashmere will be produced. The effects of genome modifications were characterized using H&E and immunohistochemistry staining, quantitative PCR, and western blotting techniques. These results indicated that the gene modifications induced by the disruption of FGF5 had occurred at the morphological and genetic levels. We further show that the knockout alleles were likely capable of germline transmission, which is essential for goat population expansion. These results provide sufficient evidences of the merit of using the CRISPR/Cas9 approach for the generation of gene-modified goats displaying the corresponding mutant phenotypes.
收录类别	SCI
语种	英语
资助项目	Provincial Key Projects of Shaanxi[2014KTDZ02-01]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000386203800025
出版者	PUBLIC LIBRARY SCIENCE
WOS关键词	GROWTH ; GENE ; GENERATION ; MUTATIONS ; REGULATOR ; LIVESTOCK ; KNOCKOUT ; LENGTH ; SYSTEM ; CELLS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1667
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Chen, Yulin
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 2.Nanjing Univ, Natl Resource Ctr Mutant Mice, MOE Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210061, Jiangsu, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Shaanxi Prov Engn & Technol Res Ctr Cashmere Goat, Yulin 719000, Peoples R China 5.Yulin Univ, Life Sci Res Ctr, Yulin 719000, Peoples R China 6.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaolong,Cai, Bei,Zhou, Jiankui,et al. Disruption of FGF5 in Cashmere Goats Using CRISPR/Cas9 Results in More Secondary Hair Follicles and Longer Fibers[J]. PLOS ONE,2016,11(10).
APA	Wang, Xiaolong.,Cai, Bei.,Zhou, Jiankui.,Zhu, Haijing.,Niu, Yiyuan.,...&Chen, Yulin.(2016).Disruption of FGF5 in Cashmere Goats Using CRISPR/Cas9 Results in More Secondary Hair Follicles and Longer Fibers.PLOS ONE,11(10).
MLA	Wang, Xiaolong,et al."Disruption of FGF5 in Cashmere Goats Using CRISPR/Cas9 Results in More Secondary Hair Follicles and Longer Fibers".PLOS ONE 11.10(2016).