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Small proline-rich proteins (SPRRs) are epidermally produced antimicrobial proteins that defend the cutaneous barrier by direct bacterial membrane disruption | |
2022-03 | |
发表期刊 | ELIFE (IF:6.4[JCR-2023],7.2[5-Year]) |
ISSN | 2050-084X |
卷号 | 11 |
发表状态 | 已发表 |
DOI | 10.7554/eLife.76729 |
摘要 | Human skin functions as a physical barrier, preventing the entry of foreign pathogens while also accommodating a myriad of commensal microorganisms. A key contributor to the skin landscape is the sebaceous gland. Mice devoid of sebocytes are prone to skin infection, yet our understanding of how sebocytes function in host defense is incomplete. Here, we show that the small proline-rich proteins, SPRR1 and SPRR2 are bactericidal in skin. SPRR1B and SPPR2A were induced in human sebocytes by exposure to the bacterial cell wall component lipopolysaccharide (LPS). Colonization of germ-free mice was insufficient to trigger increased SPRR expression in mouse skin, but LPS injected into mouse skin stimulated increased expression of the mouse SPRR orthologous genes, Sprr1a and Sprr2a, through activation of MYD88. Both mouse and human SPRR proteins displayed potent bactericidal activity against MRSA (methicillin-resistant Staphylococcus aureus), Pseudomonas aeruginosa, and skin commensals. Thus, Sprr1a(-/-);Sprr2a(-/-) mice are more susceptible to MRSA and P. aeruginosa skin infection. Lastly, mechanistic studies demonstrate that SPRR proteins exert their bactericidal activity through binding and disruption of the bacterial membrane. Taken together, these findings provide insight into the regulation and antimicrobial function of SPRR proteins in skin and how the skin defends the host against systemic infection. |
关键词 | skin innate immunity antimicrobial proteins LPS sebaceous glands Human Mouse Other |
URL | 查看原文 |
收录类别 | SCI ; SCIE |
语种 | 英语 |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
WOS类目 | Biology |
WOS记录号 | WOS:000770854300001 |
出版者 | eLIFE SCIENCES PUBL LTD |
原始文献类型 | Article |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/162918 |
专题 | 生命科学与技术学院_PI研究组_张晨璐组 |
通讯作者 | Zhang, Chenlu; Harris-Tryon, Tamia A. |
作者单位 | 1.Univ Texas Southwestern Med Ctr Dallas, Dept Dermatol, Dallas, TX 75390 USA 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 3.Univ Texas Southwestern Med Ctr Dallas, Dept Immunol, Dallas, TX 75390 USA 4.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Sch Life Sci & Biotechnol, Shanghai, Peoples R China 5.Brandenburg Med Sch Theodore Fontane, Dept Dermatol Venereol Allergol & Immunol, Dessau Med Ctr, Dessau, Germany 6.Fac Hlth Sci Brandenburg, Dessau, Germany |
第一作者单位 | 生命科学与技术学院 |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Zhang, Chenlu,Hu, Zehan,Lone, Abdul G.,et al. Small proline-rich proteins (SPRRs) are epidermally produced antimicrobial proteins that defend the cutaneous barrier by direct bacterial membrane disruption[J]. ELIFE,2022,11. |
APA | Zhang, Chenlu.,Hu, Zehan.,Lone, Abdul G..,Artami, Methinee.,Edwards, Marshall.,...&Kim, Brian S..(2022).Small proline-rich proteins (SPRRs) are epidermally produced antimicrobial proteins that defend the cutaneous barrier by direct bacterial membrane disruption.ELIFE,11. |
MLA | Zhang, Chenlu,et al."Small proline-rich proteins (SPRRs) are epidermally produced antimicrobial proteins that defend the cutaneous barrier by direct bacterial membrane disruption".ELIFE 11(2022). |
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