Clonal analysis of lineage fate in native haematopoiesis

doi:10.1038/nature25168

	Clonal analysis of lineage fate in native haematopoiesis
	Rodriguez-Fraticelli, Alejo E.1,2; Wolock, Samuel L.3; Weinreb, Caleb S.3; Panero, Riccardo 4; Patel, Sachin H.1; Jankovic, Maja 1; Sun, Jianlong1,2,5 ; Calogero, Raffaele A.4; Klein, Allon M.3; Camargo, Fernando D.1,2
	2018-01-11
发表期刊	NATURE (IF:50.5[JCR-2023],54.4[5-Year])
ISSN	0028-0836
卷号	553 期号:7687 页码:212-+
发表状态	已发表
DOI	10.1038/nature25168
摘要	Haematopoiesis, the process of mature blood and immune cell production, is functionally organized as a hierarchy, with self-renewing haematopoietic stem cells and multipotent progenitor cells sitting at the very top(1,2). Multiple models have been proposed as to what the earliest lineage choices are in these primitive haematopoietic compartments, the cellular intermediates, and the resulting lineage trees that emerge from them(3-10). Given that the bulk of studies addressing lineage outcomes have been performed in the context of haematopoietic transplantation, current models of lineage branching are more likely to represent roadmaps of lineage potential than native fate. Here we use transposon tagging to clonally trace the fates of progenitors and stem cells in unperturbed haematopoiesis. Our results describe a distinct clonal roadmap in which the megakaryocyte lineage arises largely independently of other haematopoietic fates. Our data, combined with single-cell RNA sequencing, identify a functional hierarchy of unilineage- and oligolineage-producing clones within the multipotent progenitor population. Finally, our results demonstrate that traditionally defined long-term haematopoietic stem cells are a significant source of megakaryocyte-restricted progenitors, suggesting that the megakaryocyte lineage is the predominant native fate of long-term haematopoietic stem cells. Our study provides evidence for a substantially revised roadmap for unperturbed haematopoiesis, and highlights unique properties of multipotent progenitors and haematopoietic stem cells in situ.
收录类别	SCI ; SCIE
语种	英语
资助项目	National Institutes of Health[HL128850-01A1] ; National Institutes of Health[P01HL13147]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000419794800038
出版者	NATURE PUBLISHING GROUP
WOS关键词	SINGLE-CELL TRANSCRIPTOMICS ; STEM-CELLS ; MULTIPOTENT PROGENITORS ; IDENTIFICATION ; COMMITMENT ; MEGAKARYOCYTES ; CLASSIFICATION ; SUBPOPULATIONS ; HETEROGENEITY ; HIERARCHY
原始文献类型	Article
引用统计	被引频次：386[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16253
专题	生命科学与技术学院_PI研究组_孙建龙组
通讯作者	Camargo, Fernando D.
作者单位	1.Boston Childrens Hosp, Stem Cell Program, Boston, MA 02115 USA 2.Harvard Univ, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA 3.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA 4.Univ Torino, Dept Clin & Biol Sci, Ctr Mol Biotechnol, I-10126 Turin, Italy 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Rodriguez-Fraticelli, Alejo E.,Wolock, Samuel L.,Weinreb, Caleb S.,et al. Clonal analysis of lineage fate in native haematopoiesis[J]. NATURE,2018,553(7687):212-+.
APA	Rodriguez-Fraticelli, Alejo E..,Wolock, Samuel L..,Weinreb, Caleb S..,Panero, Riccardo.,Patel, Sachin H..,...&Camargo, Fernando D..(2018).Clonal analysis of lineage fate in native haematopoiesis.NATURE,553(7687),212-+.
MLA	Rodriguez-Fraticelli, Alejo E.,et al."Clonal analysis of lineage fate in native haematopoiesis".NATURE 553.7687(2018):212-+.