Structural basis for the functional role of the Shu complex in homologous recombination

doi:10.1093/nar/gkx992

	Structural basis for the functional role of the Shu complex in homologous recombination
	Zhang, Shicheng 1; Wang, Linlin 2; Tao, Ye 1; Bai, Tuya 1; Lu, Rong1,3 ; Zhang, Tianlong 1; Chen, Jiangye 1; Ding, Jianping1,3
	2017-12-15
发表期刊	NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year])
ISSN	0305-1048
卷号	45 期号:22
发表状态	已发表
DOI	10.1093/nar/gkx992
摘要	The Shu complex, a conserved regulator consisting of Csm2, Psy3, Shu1 and Shu2 in budding yeast, plays an important role in the assembly of the Rad51-ssDNA filament in homologous recombination. However, the molecular basis for the assembly of the Shu complex and its functional role in DNA repair is still elusive. Here, we report the crystal structure of the yeast Shu complex, revealing that Csm2, Psy3, Shu1 and Shu2 interact with each other in sequence to form a V-shape overall structure. Shu1 adopts a structure resembling the ATPase core domain of Rad51 and represents a new Rad51 paralog. Shu2 assumes a novel structural fold consisting of a conserved zinc-finger containing SWIM domain and a small insertion domain. The functional roles of the key residues are validated using mutagenesis and in vitro pull-down and in vivo yeast growth studies. Structural analysis together with available biological data identifies two potential DNA-binding sites, one of which might be responsible for binding the ssDNA region of the 3'-overhang DNA and the other for the dsDNA region. Collectively, these findings reveal the molecular basis for the assembly of the Shu complex and shed new insight on its functional role in homologous recombination.
收录类别	SCI
语种	英语
资助项目	Ministry of Science and Technology of China[2013CB910404]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000419064400043
出版者	OXFORD UNIV PRESS
WOS关键词	SACCHAROMYCES-CEREVISIAE ; DNA-REPAIR ; GENES ; RAD51 ; YEAST ; PROTEINS ; SCREEN ; REPLICATION ; PARALOGS ; DOMAIN
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16252
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_丁建平组生命科学与技术学院_硕士生
通讯作者	Ding, Jianping
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci,State Key Lab Mol Biol, Shanghai Inst Biochem & Cell Biol,Natl Ctr Prot S, Shanghai Sci Res Ctr,CAS Ctr Excellence Mol Cell, 333 Haike Rd, Shanghai 201210, Peoples R China 2.Shanghai Univ, Sch Life Sci, 333 Nanchen Rd, Shanghai 200444, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Shicheng,Wang, Linlin,Tao, Ye,et al. Structural basis for the functional role of the Shu complex in homologous recombination[J]. NUCLEIC ACIDS RESEARCH,2017,45(22).
APA	Zhang, Shicheng.,Wang, Linlin.,Tao, Ye.,Bai, Tuya.,Lu, Rong.,...&Ding, Jianping.(2017).Structural basis for the functional role of the Shu complex in homologous recombination.NUCLEIC ACIDS RESEARCH,45(22).
MLA	Zhang, Shicheng,et al."Structural basis for the functional role of the Shu complex in homologous recombination".NUCLEIC ACIDS RESEARCH 45.22(2017).