Genetic targeting of Purkinje fibres by Sema3a-CreERT2

doi:10.1038/s41598-018-20829-9

	Genetic targeting of Purkinje fibres by Sema3a-CreERT2
	Li, Yan 1,2; Tian, Xueying 1,2; Zhao, Huan 1,2; He, Lingjuan 1,2; Zhang, Shaohua 1,2; Huang, Xiuzhen 1,2; Zhang, Hui 1,2; Miquerol, Lucile 3; Zhou, Bin1,2,4,5
	2018-02-05
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	8
发表状态	已发表
DOI	10.1038/s41598-018-20829-9
摘要	The maintenance of the heart rhythm and the conduction of excitatory signals require changing excitatory signals via electrical activity and coordination by communication between working and conductive cardiomyocytes. Understanding how the ventricular conduction system is established provides novel insights into the pathophysiological progress of cardiac arrhythmias. However, the major hurdle in this field is the lack of a specific genetic tool that targets the Purkinje fibres of the ventricular conduction system and no other types of cardiomyocytes or coronary vessels. Here, we generated a Sema3a-CreERT2 knock-in mouse line to test its specificity for genetically labelled Purkinje fibres. We found that Sema3a was expressed in the subendocardial layer of the trabecular myocardium in the embryonic heart and was restricted to the Purkinje fibres in the adult heart. A fate mapping study based on the Sema3a-CreERT2 line revealed that the Sema3a(+) cardiomyocytes were restricted to the fate of Purkinje fibres in the perinatal but not the embryonic stage. Collectively, our study provides a new genetic tool, i.e., Sema3a-CreERT2, for studying the molecular mechanisms that regulate the function of Purkinje fibres.
收录类别	SCI ; SCIE
语种	英语
资助项目	Health and Medical Research Fund[03140346] ; Health and Medical Research Fund[04152566]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000424087700054
出版者	NATURE PUBLISHING GROUP
WOS关键词	SEMAPHORINS ; SYSTEM
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16238
专题	生命科学与技术学院_特聘教授组_周斌组
通讯作者	Zhou, Bin
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci Shanghai, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutr Sci, Shanghai 200031, Peoples R China 3.Aix Marseille Univ, CNRS, IBDM, UMR 7288, F-13288 Marseille, France 4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou 510632, Guangdong, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Li, Yan,Tian, Xueying,Zhao, Huan,et al. Genetic targeting of Purkinje fibres by Sema3a-CreERT2[J]. SCIENTIFIC REPORTS,2018,8.
APA	Li, Yan.,Tian, Xueying.,Zhao, Huan.,He, Lingjuan.,Zhang, Shaohua.,...&Zhou, Bin.(2018).Genetic targeting of Purkinje fibres by Sema3a-CreERT2.SCIENTIFIC REPORTS,8.
MLA	Li, Yan,et al."Genetic targeting of Purkinje fibres by Sema3a-CreERT2".SCIENTIFIC REPORTS 8(2018).