Label-free graphene biosensor targeting cancer molecules based on non-covalent modification

doi:10.1016/j.bios.2016.09.025

	Label-free graphene biosensor targeting cancer molecules based on non-covalent modification
	Zhou, Lin 1,2; Mao, Hongju 1; Wu, Chunyan 1,3; Tang, Lin1,4 ; Wu, Zhenhua 1,2; Sun, Hao 1; Zhang, Honglian 1; Zhou, Hongbo 1; Jia, Chunping 1; Jin, Qinghui 1; Chen, Xianfeng 5; Zhao, Jianlong1
	2017-01-15
发表期刊	BIOSENSORS & BIOELECTRONICS (IF:10.7[JCR-2023],9.9[5-Year])
ISSN	0956-5663
卷号	87 页码:701-707
发表状态	已发表
DOI	10.1016/j.bios.2016.09.025
摘要	A label-free immunosensor based on antibody-modified graphene field effect transistor (GFET) was presented. Antibodies targeting carcinoembryonic antigen (Anti-CEA) were immobilized to the graphene surface via non-covalent modification. The bifunctional molecule, 1-pyrenebutanoic acid succinimidyl ester, which is composed of a pyrene and a reactive succinimide ester group, interacts with graphene non-covalently via pi-stacking. The succinimide ester group reacts with the amine group to initiate antibody surface immobilization, which was confirmed by X-ray Photoelectron Spectroscopy, Atomic Force Microscopy and Electrochemical Impedance Spectroscopy. The resulting anti-CEA modified GFET sufficiently monitored the reaction between CEA protein and anti-CEA in real-time with high specificity, which revealed selective electrical detection of CEA with a limit of detection (LOD) of less than 100 pg/ml. The dissociation constant between CEA protein and anti-CEA was estimated to be 6.35 x 10(-11) M, indicating the high affinity and sensitivity of anti-CEA-GFET. Taken together, the graphene biosensors provide an effective tool for clinical application and point-of-care medical diagnostics. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Graphene Non-covalent modification CEA protein Dissociation constant
收录类别	SCI ; EI
语种	英语
资助项目	National Natural Science Foundation of China[61571429] ; National Natural Science Foundation of China[61401442] ; National Natural Science Foundation of China[61571428] ; National Natural Science Foundation of China[61571077]
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000390499600097
出版者	ELSEVIER ADVANCED TECHNOLOGY
EI入藏号	20163902833581
EI主题词	Antibodies ; Atomic force microscopy ; Biosensors ; Diagnosis ; Dissociation ; Electrochemical impedance spectroscopy ; Esters ; Field effect transistors ; Graphene ; Graphene transistors ; Molecules ; Proteins
EI分类号	Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Semiconductor Devices and Integrated Circuits:714.2 ; Optical Devices and Systems:741.3 ; Nanotechnology:761 ; Chemistry:801 ; Chemical Reactions:802.2 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Atomic and Molecular Physics:931.3
WOS关键词	FIELD-EFFECT TRANSISTORS ; CARCINOEMBRYONIC ANTIGEN ; MONOCLONAL-ANTIBODIES ; PROTEIN ; FUNCTIONALIZATION ; IMMOBILIZATION ; ADSORPTION ; BINDING
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1543
专题	信息科学与技术学院_硕士生信息科学与技术学院_特聘教授组_赵建龙组
通讯作者	Mao, Hongju; Zhao, Jianlong
作者单位	1.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Key Lab Terahertz Solid State Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Shanghai Univ, Shanghai 200031, Peoples R China 4.ShanghaiTech Univ, Shanghai 200444, Peoples R China 5.Bangor Univ, Sch Elect Engn, Bangor LL57 1UT, Gwynedd, Wales
推荐引用方式 GB/T 7714	Zhou, Lin,Mao, Hongju,Wu, Chunyan,et al. Label-free graphene biosensor targeting cancer molecules based on non-covalent modification[J]. BIOSENSORS & BIOELECTRONICS,2017,87:701-707.
APA	Zhou, Lin.,Mao, Hongju.,Wu, Chunyan.,Tang, Lin.,Wu, Zhenhua.,...&Zhao, Jianlong.(2017).Label-free graphene biosensor targeting cancer molecules based on non-covalent modification.BIOSENSORS & BIOELECTRONICS,87,701-707.
MLA	Zhou, Lin,et al."Label-free graphene biosensor targeting cancer molecules based on non-covalent modification".BIOSENSORS & BIOELECTRONICS 87(2017):701-707.