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ShanghaiTech University Knowledge Management System
Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System | |
2017-03-15 | |
发表期刊 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (IF:14.4[JCR-2023],14.8[5-Year]) |
ISSN | 0002-7863 |
卷号 | 139期号:10页码:3790-3795 |
发表状态 | 已发表 |
DOI | 10.1021/jacs.6b13317 |
摘要 | Staphylococcus aureus, a major human pathogen, has been the cause of serious infectious diseases with a high mortality rate. Although genetics is a key means to study S. aureus physiology, such as drug resistance and pathogenesis, genetic manipulation in S. aureus is always time-consuming and laborintensive. Here we report a CRISPR/Cas9 system (pCasSA) for rapid and efficient genome editing, including gene deletion, insertion, and single-base substitution mutation in S. aureus. The designed pCasSA system is amenable to the assembly of spacers and repair arms by Golden Gate assembly and Gibson assembly, respectively, enabling rapid construction of the plasmids for editing. We further engineered the pCasSA system to be an efficient transcription inhibition system for gene knockdown and possible genome-wide screening. The development of the CRISPR/Cas9-mediated genome editing and transcription inhibition tools will dramatically accelerate drug-target exploration and drug development. |
收录类别 | SCI ; EI |
语种 | 英语 |
资助项目 | NIH[AI121664] |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000396801300033 |
出版者 | AMER CHEMICAL SOC |
EI入藏号 | 20171103449062 |
EI主题词 | Bacteria ; Diagnosis ; Drug therapy ; Enzyme inhibition ; Transcription |
EI分类号 | Bioengineering and Biology:461 |
WOS关键词 | GENE-EXPRESSION ; DNA RECOGNITION ; TARGET DNA ; CAS9 ; RNA ; VIRULENCE ; ENDONUCLEASE ; SPECIFICITY ; INFECTIONS ; NUCLEASES |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1471 |
专题 | 物质科学与技术学院 物质科学与技术学院_PI研究组_季泉江组 物质科学与技术学院_硕士生 |
通讯作者 | Ji, Quanjiang |
作者单位 | 1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.Indiana Univ, Sch Med Northwest, Dept Microbiol & Immunol, Gary, IN 46408 USA |
第一作者单位 | 物质科学与技术学院 |
通讯作者单位 | 物质科学与技术学院 |
第一作者的第一单位 | 物质科学与技术学院 |
推荐引用方式 GB/T 7714 | Chen, Weizhong,Zhang, Yifei,Yeo, Won-Sik,et al. Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2017,139(10):3790-3795. |
APA | Chen, Weizhong,Zhang, Yifei,Yeo, Won-Sik,Bae, Taeok,&Ji, Quanjiang.(2017).Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,139(10),3790-3795. |
MLA | Chen, Weizhong,et al."Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139.10(2017):3790-3795. |
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