Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System

doi:10.1021/jacs.6b13317

	Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System
	Chen, Weizhong1 ; Zhang, Yifei1 ; Yeo, Won-Sik 2; Bae, Taeok 2; Ji, Quanjiang1
	2017-03-15
发表期刊	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (IF:14.4[JCR-2023],14.8[5-Year])
ISSN	0002-7863
卷号	139 期号:10 页码:3790-3795
发表状态	已发表
DOI	10.1021/jacs.6b13317
摘要	Staphylococcus aureus, a major human pathogen, has been the cause of serious infectious diseases with a high mortality rate. Although genetics is a key means to study S. aureus physiology, such as drug resistance and pathogenesis, genetic manipulation in S. aureus is always time-consuming and laborintensive. Here we report a CRISPR/Cas9 system (pCasSA) for rapid and efficient genome editing, including gene deletion, insertion, and single-base substitution mutation in S. aureus. The designed pCasSA system is amenable to the assembly of spacers and repair arms by Golden Gate assembly and Gibson assembly, respectively, enabling rapid construction of the plasmids for editing. We further engineered the pCasSA system to be an efficient transcription inhibition system for gene knockdown and possible genome-wide screening. The development of the CRISPR/Cas9-mediated genome editing and transcription inhibition tools will dramatically accelerate drug-target exploration and drug development.
收录类别	SCI ; EI
语种	英语
资助项目	NIH[AI121664]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000396801300033
出版者	AMER CHEMICAL SOC
EI入藏号	20171103449062
EI主题词	Bacteria ; Diagnosis ; Drug therapy ; Enzyme inhibition ; Transcription
EI分类号	Bioengineering and Biology:461
WOS关键词	GENE-EXPRESSION ; DNA RECOGNITION ; TARGET DNA ; CAS9 ; RNA ; VIRULENCE ; ENDONUCLEASE ; SPECIFICITY ; INFECTIONS ; NUCLEASES
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1471
专题	物质科学与技术学院物质科学与技术学院_PI研究组_季泉江组物质科学与技术学院_硕士生
通讯作者	Ji, Quanjiang
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.Indiana Univ, Sch Med Northwest, Dept Microbiol & Immunol, Gary, IN 46408 USA
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Chen, Weizhong,Zhang, Yifei,Yeo, Won-Sik,et al. Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2017,139(10):3790-3795.
APA	Chen, Weizhong,Zhang, Yifei,Yeo, Won-Sik,Bae, Taeok,&Ji, Quanjiang.(2017).Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,139(10),3790-3795.
MLA	Chen, Weizhong,et al."Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139.10(2017):3790-3795.