消息
×
loading..
Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System
2017-03-15
发表期刊JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (IF:14.4[JCR-2023],14.8[5-Year])
ISSN0002-7863
卷号139期号:10页码:3790-3795
发表状态已发表
DOI10.1021/jacs.6b13317
摘要Staphylococcus aureus, a major human pathogen, has been the cause of serious infectious diseases with a high mortality rate. Although genetics is a key means to study S. aureus physiology, such as drug resistance and pathogenesis, genetic manipulation in S. aureus is always time-consuming and laborintensive. Here we report a CRISPR/Cas9 system (pCasSA) for rapid and efficient genome editing, including gene deletion, insertion, and single-base substitution mutation in S. aureus. The designed pCasSA system is amenable to the assembly of spacers and repair arms by Golden Gate assembly and Gibson assembly, respectively, enabling rapid construction of the plasmids for editing. We further engineered the pCasSA system to be an efficient transcription inhibition system for gene knockdown and possible genome-wide screening. The development of the CRISPR/Cas9-mediated genome editing and transcription inhibition tools will dramatically accelerate drug-target exploration and drug development.
收录类别SCI ; EI
语种英语
资助项目NIH[AI121664]
WOS研究方向Chemistry
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000396801300033
出版者AMER CHEMICAL SOC
EI入藏号20171103449062
EI主题词Bacteria ; Diagnosis ; Drug therapy ; Enzyme inhibition ; Transcription
EI分类号Bioengineering and Biology:461
WOS关键词GENE-EXPRESSION ; DNA RECOGNITION ; TARGET DNA ; CAS9 ; RNA ; VIRULENCE ; ENDONUCLEASE ; SPECIFICITY ; INFECTIONS ; NUCLEASES
原始文献类型Article
引用统计
被引频次:97[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1471
专题物质科学与技术学院
物质科学与技术学院_PI研究组_季泉江组
物质科学与技术学院_硕士生
通讯作者Ji, Quanjiang
作者单位
1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
2.Indiana Univ, Sch Med Northwest, Dept Microbiol & Immunol, Gary, IN 46408 USA
第一作者单位物质科学与技术学院
通讯作者单位物质科学与技术学院
第一作者的第一单位物质科学与技术学院
推荐引用方式
GB/T 7714
Chen, Weizhong,Zhang, Yifei,Yeo, Won-Sik,et al. Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2017,139(10):3790-3795.
APA Chen, Weizhong,Zhang, Yifei,Yeo, Won-Sik,Bae, Taeok,&Ji, Quanjiang.(2017).Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,139(10),3790-3795.
MLA Chen, Weizhong,et al."Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139.10(2017):3790-3795.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Chen, Weizhong]的文章
[Zhang, Yifei]的文章
[Yeo, Won-Sik]的文章
百度学术
百度学术中相似的文章
[Chen, Weizhong]的文章
[Zhang, Yifei]的文章
[Yeo, Won-Sik]的文章
必应学术
必应学术中相似的文章
[Chen, Weizhong]的文章
[Zhang, Yifei]的文章
[Yeo, Won-Sik]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1471.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。