Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting

doi:10.1038/srep45169

	Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting
	Luo, Si-Wei 1,2,3; Liang, Zhi 2,3; Wu, Jia-Rui1,2,3,4
	2017-03-24
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7
发表状态	已发表
DOI	10.1038/srep45169
摘要	Quantitatively detecting correlations of multiple protein-protein interactions (PPIs) in vivo is a big challenge. Here we introduce a novel method, termed Protein-interactome Footprinting (PiF), to simultaneously measure multiple PPIs in one cell. The principle of PiF is that each target physical PPI in the interactome is simultaneously transcoded into a specific DNA sequence based on dimerization of the target proteins fused with DNA-binding domains. The interaction intensity of each target protein is quantified as the copy number of the specific DNA sequences bound by each fusion protein dimers. Using PiF, we quantitatively reveal dynamic patterns of PPIs and their correlation network in E. coli twocomponent systems.
收录类别	SCI
语种	英语
资助项目	Ministry of Science and Technology[2012CB917200] ; Ministry of Science and Technology[2014CB910600]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000397381000001
出版者	NATURE PUBLISHING GROUP
WOS关键词	SIGNAL-TRANSDUCTION ; ESCHERICHIA-COLI ; WEB SERVER ; PHOSPHORYLATION ; NETWORK ; BINDING ; SYSTEMS ; CHEA ; MAP
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1458
专题	生命科学与技术学院
通讯作者	Liang, Zhi; Wu, Jia-Rui
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai, Peoples R China 2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Luo, Si-Wei,Liang, Zhi,Wu, Jia-Rui. Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting[J]. SCIENTIFIC REPORTS,2017,7.
APA	Luo, Si-Wei,Liang, Zhi,&Wu, Jia-Rui.(2017).Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting.SCIENTIFIC REPORTS,7.
MLA	Luo, Si-Wei,et al."Simultaneously measuring multiple protein interactions and their correlations in a cell by Protein-interactome Footprinting".SCIENTIFIC REPORTS 7(2017).