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Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber
2018-02
发表期刊COLLOIDS AND SURFACES B-BIOINTERFACES (IF:5.4[JCR-2023],5.1[5-Year])
ISSN0927-7765
卷号162页码:390-397
发表状态已发表
DOI10.1016/j.colsurfb.2017.12.010
摘要Epithelial-mesenchymal transition (EMT) is a process by which epithelial cells lose their cell cell contact to become mesenchymal stem cells, which is important on development and embryogenesis, wound healing, and cancer metastasis. This research aims to investigate the effect of topological cue as modulating factor on the EMT by tuning the diameter of electrospinning nanofiber. The cell-nanofiber interaction between human lung epithelial cell A549 and electrospinning nanofibers composed of polyvinyl alcohol (PVA) and type I collagen were investigated. The electrospinning of regenerated PVA/Collagen nanofibers were performed with water/acetic acid as a spinning solvent and glutaraldehyde as a chemical cross linker. Parameterization on concentration, applied voltage and feeding rate was finalized to generate smooth nanofibers with good homogeneity. The scanning electron microscopy result demonstrated that A549 cell appropriately achieved extended morphology by the filopodia attaching to the surface of the nanofibrous mats. When the diameter changed from 90 nm to 240 nm, the A549 cell was correspondingly express varied EMT related genes. Gene expression analysis was conducted by qPCR using three typical markers for detecting EMT: N-cadherin (NCad), Vimentin (Vim), and Fibronectin (Fib). An increasing expression pattern was observed on cell culturing on 170 nm sample with respect to cell cultured on 90 nm and 240 nm. This result indicated the 170 nm PVA/Collagen nanofibers induce A549 cells to process epithelial mesenchymal transition more seriously than those on 90 nm or 240 nm. (C) 2017 Elsevier B.V. All rights reserved.
关键词Electrospinning Nanofiber PVA/Collagen A549 cell Epithelial-mesenchymal transition
收录类别SCI ; SCIE ; EI
语种英语
WOS研究方向Biophysics ; Chemistry ; Materials Science
WOS类目Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
WOS记录号WOS:000425076500046
出版者ELSEVIER SCIENCE BV
EI入藏号20175004538540
EI主题词Biological organs ; Cell culture ; Cytology ; Diseases ; Electrospinning ; Gene expression ; Genes ; Nanofibers ; Polymerase chain reaction ; Respiratory system ; Scanning electron microscopy ; Spinning (fibers) ; Stem cells ; Tissue
EI分类号Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Nanotechnology:761 ; Biochemistry:801.2 ; Fiber Chemistry and Processing:819.3 ; Solid State Physics:933
WOS关键词METASTASIS ; PHENOTYPE ; CULTURE ; CANCER
原始文献类型Article
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/14275
专题物质科学与技术学院
物质科学与技术学院_PI研究组_龚晋慷组
生命科学与技术学院_PI研究组_孙建龙组
附属学校、幼儿园筹建部
物质科学与技术学院_硕士生
物质科学与技术学院_博士生
通讯作者Gong, Jinkang; Yao, Yuan
作者单位
1.ShanghaiTech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Ceram, 1295 Dingxi Rd, Shanghai 200050, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Organ Chem, 345 Lingling Lu, Shanghai 200032, Peoples R China
4.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China
第一作者单位物质科学与技术学院
通讯作者单位物质科学与技术学院
第一作者的第一单位物质科学与技术学院
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GB/T 7714
Li, Xiuchun,Yan, Shanshan,Dai, Jing,et al. Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2018,162:390-397.
APA Li, Xiuchun.,Yan, Shanshan.,Dai, Jing.,Lu, Yi.,Wang, Yiqun.,...&Yao, Yuan.(2018).Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber.COLLOIDS AND SURFACES B-BIOINTERFACES,162,390-397.
MLA Li, Xiuchun,et al."Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber".COLLOIDS AND SURFACES B-BIOINTERFACES 162(2018):390-397.
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