Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics

doi:10.1038/s41598-017-00841-1

	Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics
	Tang, Jia-shu 1; Li, Qing-run 1; Li, Jia-ming 1; Wu, Jia-rui1,2 ; Zeng, Rong1,2
	2017-04-21
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7
发表状态	已发表
DOI	10.1038/s41598-017-00841-1
摘要	GLP-1 synergizes with glucose in regulating pancreatic beta-cell function, including facilitating beta-cell survival and insulin secretion. Though it has been widely accepted that phosphorylation is extremely important in regulating beta-cell functions, our knowledge to the global mechanism is still limited. Here we performed a quantitative phosphoproteomics study to systematically present the synergistic regulation of INS-1E cell phosphoproteome mediated by glucose and GLP-1. We generated the largest pancreatic beta-cell phosphoproteome by identifying 25,327 accurately localized phosphorylation sites on 5,389 proteins. Our results discovered several novel kinases regulated by glucose, GLP-1 or their synergism, and some of these kinases might act as downstream molecules of GLP-1 mediated PKA signaling cascade. A few phosphosites were regulated by both GLP-1 and glucose alone, and these target proteins were highly related to their biological function on pancreatic beta-cells. Finally, we found glucose and GLP-1 executed their synergistic effect at multiple levels, especially at pathway level. Both GLP-1 and glucose participated in regulating every single step of the secretion pathway, and systematically synergized their effects in inducing insulin secretion.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[91539124] ; National Natural Science Foundation of China[31130034]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000399972900017
出版者	NATURE PUBLISHING GROUP
WOS关键词	PANCREATIC BETA-CELLS ; ACTIVATED PROTEIN-KINASE ; SITE-SPECIFIC PHOSPHORYLATION ; GLUCAGON-LIKE PEPTIDE-1 ; ACETYL-COA CARBOXYLASE ; LARGE GENE LISTS ; MASS-SPECTROMETRY ; IN-VIVO ; CAMP ; EXOCYTOSIS
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1407
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_曾嵘组
通讯作者	Wu, Jia-rui; Zeng, Rong
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Dept Life Sci, 99 Haike Rd, Shanghai 201210, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,et al. Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics[J]. SCIENTIFIC REPORTS,2017,7.
APA	Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,Wu, Jia-rui,&Zeng, Rong.(2017).Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics.SCIENTIFIC REPORTS,7.
MLA	Tang, Jia-shu,et al."Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics".SCIENTIFIC REPORTS 7(2017).