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| Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics | |
| 2017-04-21 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 7 |
| Status | 已发表 |
| DOI | 10.1038/s41598-017-00841-1 |
| Abstract | GLP-1 synergizes with glucose in regulating pancreatic beta-cell function, including facilitating beta-cell survival and insulin secretion. Though it has been widely accepted that phosphorylation is extremely important in regulating beta-cell functions, our knowledge to the global mechanism is still limited. Here we performed a quantitative phosphoproteomics study to systematically present the synergistic regulation of INS-1E cell phosphoproteome mediated by glucose and GLP-1. We generated the largest pancreatic beta-cell phosphoproteome by identifying 25,327 accurately localized phosphorylation sites on 5,389 proteins. Our results discovered several novel kinases regulated by glucose, GLP-1 or their synergism, and some of these kinases might act as downstream molecules of GLP-1 mediated PKA signaling cascade. A few phosphosites were regulated by both GLP-1 and glucose alone, and these target proteins were highly related to their biological function on pancreatic beta-cells. Finally, we found glucose and GLP-1 executed their synergistic effect at multiple levels, especially at pathway level. Both GLP-1 and glucose participated in regulating every single step of the secretion pathway, and systematically synergized their effects in inducing insulin secretion. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[91539124] ; National Natural Science Foundation of China[31130034] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000399972900017 |
| Publisher | NATURE PUBLISHING GROUP |
| WOS Keyword | PANCREATIC BETA-CELLS ; ACTIVATED PROTEIN-KINASE ; SITE-SPECIFIC PHOSPHORYLATION ; GLUCAGON-LIKE PEPTIDE-1 ; ACETYL-COA CARBOXYLASE ; LARGE GENE LISTS ; MASS-SPECTROMETRY ; IN-VIVO ; CAMP ; EXOCYTOSIS |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1407 |
| Collection | 生命科学与技术学院 生命科学与技术学院_特聘教授组_曾嵘组 |
| Corresponding Author | Wu, Jia-rui; Zeng, Rong |
| Affiliation | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Dept Life Sci, 99 Haike Rd, Shanghai 201210, Peoples R China |
| Corresponding Author Affilication | ShanghaiTech University |
| Recommended Citation GB/T 7714 | Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,et al. Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,Wu, Jia-rui,&Zeng, Rong.(2017).Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics.SCIENTIFIC REPORTS,7. |
| MLA | Tang, Jia-shu,et al."Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics".SCIENTIFIC REPORTS 7(2017). |
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