Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics
2017-04-21
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume7
Status已发表
DOI10.1038/s41598-017-00841-1
AbstractGLP-1 synergizes with glucose in regulating pancreatic beta-cell function, including facilitating beta-cell survival and insulin secretion. Though it has been widely accepted that phosphorylation is extremely important in regulating beta-cell functions, our knowledge to the global mechanism is still limited. Here we performed a quantitative phosphoproteomics study to systematically present the synergistic regulation of INS-1E cell phosphoproteome mediated by glucose and GLP-1. We generated the largest pancreatic beta-cell phosphoproteome by identifying 25,327 accurately localized phosphorylation sites on 5,389 proteins. Our results discovered several novel kinases regulated by glucose, GLP-1 or their synergism, and some of these kinases might act as downstream molecules of GLP-1 mediated PKA signaling cascade. A few phosphosites were regulated by both GLP-1 and glucose alone, and these target proteins were highly related to their biological function on pancreatic beta-cells. Finally, we found glucose and GLP-1 executed their synergistic effect at multiple levels, especially at pathway level. Both GLP-1 and glucose participated in regulating every single step of the secretion pathway, and systematically synergized their effects in inducing insulin secretion.
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[91539124] ; National Natural Science Foundation of China[31130034]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000399972900017
PublisherNATURE PUBLISHING GROUP
WOS KeywordPANCREATIC BETA-CELLS ; ACTIVATED PROTEIN-KINASE ; SITE-SPECIFIC PHOSPHORYLATION ; GLUCAGON-LIKE PEPTIDE-1 ; ACETYL-COA CARBOXYLASE ; LARGE GENE LISTS ; MASS-SPECTROMETRY ; IN-VIVO ; CAMP ; EXOCYTOSIS
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1407
Collection生命科学与技术学院
生命科学与技术学院_特聘教授组_曾嵘组
Corresponding AuthorWu, Jia-rui; Zeng, Rong
Affiliation
1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China
2.ShanghaiTech Univ, Dept Life Sci, 99 Haike Rd, Shanghai 201210, Peoples R China
Corresponding Author AffilicationShanghaiTech University
Recommended Citation
GB/T 7714
Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,et al. Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics[J]. SCIENTIFIC REPORTS,2017,7.
APA Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,Wu, Jia-rui,&Zeng, Rong.(2017).Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics.SCIENTIFIC REPORTS,7.
MLA Tang, Jia-shu,et al."Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics".SCIENTIFIC REPORTS 7(2017).
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