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ShanghaiTech University Knowledge Management System
Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics | |
2017-04-21 | |
发表期刊 | SCIENTIFIC REPORTS (IF:3.8[JCR-2023],4.3[5-Year]) |
ISSN | 2045-2322 |
卷号 | 7 |
发表状态 | 已发表 |
DOI | 10.1038/s41598-017-00841-1 |
摘要 | GLP-1 synergizes with glucose in regulating pancreatic beta-cell function, including facilitating beta-cell survival and insulin secretion. Though it has been widely accepted that phosphorylation is extremely important in regulating beta-cell functions, our knowledge to the global mechanism is still limited. Here we performed a quantitative phosphoproteomics study to systematically present the synergistic regulation of INS-1E cell phosphoproteome mediated by glucose and GLP-1. We generated the largest pancreatic beta-cell phosphoproteome by identifying 25,327 accurately localized phosphorylation sites on 5,389 proteins. Our results discovered several novel kinases regulated by glucose, GLP-1 or their synergism, and some of these kinases might act as downstream molecules of GLP-1 mediated PKA signaling cascade. A few phosphosites were regulated by both GLP-1 and glucose alone, and these target proteins were highly related to their biological function on pancreatic beta-cells. Finally, we found glucose and GLP-1 executed their synergistic effect at multiple levels, especially at pathway level. Both GLP-1 and glucose participated in regulating every single step of the secretion pathway, and systematically synergized their effects in inducing insulin secretion. |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[91539124] ; National Natural Science Foundation of China[31130034] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000399972900017 |
出版者 | NATURE PUBLISHING GROUP |
WOS关键词 | PANCREATIC BETA-CELLS ; ACTIVATED PROTEIN-KINASE ; SITE-SPECIFIC PHOSPHORYLATION ; GLUCAGON-LIKE PEPTIDE-1 ; ACETYL-COA CARBOXYLASE ; LARGE GENE LISTS ; MASS-SPECTROMETRY ; IN-VIVO ; CAMP ; EXOCYTOSIS |
原始文献类型 | Article |
引用统计 | 正在获取...
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文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1407 |
专题 | 生命科学与技术学院 生命科学与技术学院_特聘教授组_曾嵘组 |
通讯作者 | Wu, Jia-rui; Zeng, Rong |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Dept Life Sci, 99 Haike Rd, Shanghai 201210, Peoples R China |
通讯作者单位 | 上海科技大学 |
推荐引用方式 GB/T 7714 | Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,et al. Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics[J]. SCIENTIFIC REPORTS,2017,7. |
APA | Tang, Jia-shu,Li, Qing-run,Li, Jia-ming,Wu, Jia-rui,&Zeng, Rong.(2017).Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics.SCIENTIFIC REPORTS,7. |
MLA | Tang, Jia-shu,et al."Systematic Synergy of Glucose and GLP-1 to Stimulate Insulin Secretion Revealed by Quantitative Phosphoproteomics".SCIENTIFIC REPORTS 7(2017). |
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