Understanding lncRNA–protein assemblies with imaging and single-molecule approaches

doi:10.1016/j.gde.2021.11.005

	Understanding lncRNA–protein assemblies with imaging and single-molecule approaches
	Liu, Jiaquan 1; Yang, Liang-Zhong 1; Chen, Ling-Ling1,2,3
	2022-02
发表期刊	CURRENT OPINION IN GENETICS & DEVELOPMENT (IF:3.7[JCR-2023],4.1[5-Year])
ISSN	0959-437X
EISSN	1879-0380
卷号	72 页码:128-137
发表状态	已发表
DOI	10.1016/j.gde.2021.11.005
摘要	Long non-coding RNAs (lncRNAs) associate with RNA-binding proteins (RBPs) to form lncRNA–protein complexes that act in a wide range of biological processes. Understanding the molecular mechanism of how a lncRNA–protein complex is assembled and regulated is key for their cellular functions. In this mini-review, we outline molecular methods used to identify lncRNA–protein interactions from large-scale to individual levels using bulk cells as well as those recently developed imaging and single-molecule approaches that are capable of visualizing RNA–protein assemblies in single cells and in real-time. Focusing on the latter group of approaches, we discuss their applications and limitations, which nevertheless have enabled quantification and comprehensive dissection of RNA–protein interactions possible.
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收录类别	SCIE ; SCI
语种	英语
资助项目	Chinese Academy of Sciences (CAS)[YSBR-009] ; Shanghai Municipal Commission for Science and Technology[20JC1410300] ; National Natural Science Foundation of China (NSFC)[31725009,31830108] ; HHMI International Program[55008728] ; NSFC[32071283] ; Natural Science Foundation of Shanghai[20ZR1474100] ; Shanghai Pujiang Program[20PJ1414500]
WOS研究方向	Cell Biology ; Genetics & Heredity
WOS类目	Cell Biology ; Genetics & Heredity
WOS记录号	WOS:000760311500016
出版者	CURRENT BIOLOGY LTD
Scopus 记录号	2-s2.0-85121429261
来源库	Scopus
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/139005
专题	生命科学与技术学院_特聘教授组_陈玲玲组
通讯作者	Liu, Jiaquan; Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab M, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Jiaquan,Yang, Liang-Zhong,Chen, Ling-Ling. Understanding lncRNA–protein assemblies with imaging and single-molecule approaches[J]. CURRENT OPINION IN GENETICS & DEVELOPMENT,2022,72:128-137.
APA	Liu, Jiaquan,Yang, Liang-Zhong,&Chen, Ling-Ling.(2022).Understanding lncRNA–protein assemblies with imaging and single-molecule approaches.CURRENT OPINION IN GENETICS & DEVELOPMENT,72,128-137.
MLA	Liu, Jiaquan,et al."Understanding lncRNA–protein assemblies with imaging and single-molecule approaches".CURRENT OPINION IN GENETICS & DEVELOPMENT 72(2022):128-137.