Metabolomic and Proteomic Analyses of Persistent Valvular Atrial Fibrillation and Non-Valvular Atrial Fibrillation

doi:10.3389/fgene.2021.789485

	Metabolomic and Proteomic Analyses of Persistent Valvular Atrial Fibrillation and Non-Valvular Atrial Fibrillation
	Hu, Bo 1; Ge, Wen 2; Wang, Yuliang 3; Zhang, Xiaobin 4; Li, Tao 5; Cui, Hui6 ; Qian, Yongjun 5; Zhang, Yangyang 4; Li, Zhi 7
	2021-11-30
发表期刊	FRONTIERS IN GENETICS (IF:2.8[JCR-2023],3.3[5-Year])
EISSN	1664-8021
卷号	12
发表状态	已发表
DOI	10.3389/fgene.2021.789485
摘要	Atrial fibrillation (AF) is an abnormal heart rhythm related to an increased risk of heart failure, dementia, and stroke. The distinction between valvular and non-valvular AF remains a debate. In this study, proteomics and metabolomics were integrated to describe the dysregulated metabolites and proteins of AF patients relative to sinus rhythm (SR) patients. Totally 47 up-regulated and 41 down-regulated proteins in valvular AF, and 59 up-regulated and 149 down-regulated proteins in non-valvular AF were recognized in comparison to SR patients. Moreover, 58 up-regulated and 49 significantly down-regulated metabolites in valvular AF, and 47 up-regulated and 122 down-regulated metabolites in persistent non-valvular AF patients were identified in comparison to SR patients. Based on analysis of differential levels of metabolites and proteins, 15 up-regulated and 22 down-regulated proteins, and 13 up-regulated and 122 down-regulated metabolites in persistent non-valvular AF were identified relative to valvular AF. KEGG pathway enrichment analysis showed the altered proteins and metabolites were significantly related to multiple metabolic pathways, such as Glycolysis/Gluconeogenesis. Interestingly, the enrichment pathways related to non-valvular AF were obviously different from those in valvular AF. For example, valvular AF was significantly related to Glycolysis/Gluconeogenesis, but non-valvular AF was more related to Citrate cycle (TCA cycle). Correlation analysis between the differentially expressed proteins and metabolites was also performed. Several hub proteins with metabolites were identified in valvular AF and non-valvular AF. For example, Taurine, D-Threitol, L-Rhamnose, and DL-lactate played crucial roles in valvular AF, while Glycerol-3-phosphate dehydrogenase, Inorganic pyrophosphatase 2, Hydroxymethylglutaryl-CoAlyase, and Deoxyuridine 5-triphosphate nucleotidohydrolase were crucial in non-valvular AF. Then two hub networks were recognized as potential biomarkers, which can effectively distinguish valvular AF and non-valvular persistent AF from SR samples, with areas under curve of 0.75 and 0.707, respectively. Hence, these metabolites and proteins can be used as potential clinical molecular markers to discriminate two types of AF from SR samples. In summary, this study provides novel insights to understanding the mechanisms of AF progression and identifying novel biomarkers for prognosis of non-valvular AF and valvular AF by using metabolomics and proteomics analyses.
关键词	metabolic proteomic valvular atrial fibrillation non-valvular atrial fibrillation persistent atrial fibrillation
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收录类别	SCI ; SCIE
语种	英语
资助项目	Scientific Research Project of National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University[Z2018B19] ; Jiangsu Province Hospital Clinical Improvement Program[JSPHMB-2020-4] ; 1.3 project for disciplines of excellence-Clinical Research Incubation Project, West China Hospital, Sichuan University[2019HXFH029] ; Technology innovation research and development project of Chengdu Science and Technology Bureau[2019-YF05-00183-SN] ; Major Science and Technology Project of Sichuan Province, China[2021YFS0121]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000729898800001
出版者	FRONTIERS MEDIA SA
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/138714
专题	生命科学与技术学院_博士生
通讯作者	Qian, Yongjun; Zhang, Yangyang; Li, Zhi
作者单位	1.Tongji Univ, Shanghai East Hosp, Sch Med, Dept Cardiol, Shanghai, Peoples R China 2.Shanghai Univ TCM, Shuguang Hosp, Dept Cardiothorac Surg, Shanghai, Peoples R China 3.Nanjing Med Univ, Dept Immunol, Nanjing, Peoples R China 4.Shanghai Jiao Tong Univ, Shanghai Chest Hosp, Dept Cardiovasc Surg, Shanghai, Peoples R China 5.Sichuan Univ, West China Hosp, Natl Clin Res Ctr Geriatr, Dept Cardiovasc Surg, Chengdu, Peoples R China 6.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 7.Nanjing Med Univ, Jiangsu Prov Hosp, Dept Cardiovasc Surg, Affiliated Hosp 1, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Hu, Bo,Ge, Wen,Wang, Yuliang,et al. Metabolomic and Proteomic Analyses of Persistent Valvular Atrial Fibrillation and Non-Valvular Atrial Fibrillation[J]. FRONTIERS IN GENETICS,2021,12.
APA	Hu, Bo.,Ge, Wen.,Wang, Yuliang.,Zhang, Xiaobin.,Li, Tao.,...&Li, Zhi.(2021).Metabolomic and Proteomic Analyses of Persistent Valvular Atrial Fibrillation and Non-Valvular Atrial Fibrillation.FRONTIERS IN GENETICS,12.
MLA	Hu, Bo,et al."Metabolomic and Proteomic Analyses of Persistent Valvular Atrial Fibrillation and Non-Valvular Atrial Fibrillation".FRONTIERS IN GENETICS 12(2021).