Both Clathrin-Mediated and Membrane Microdomain-Associated Endocytosis Contribute to the Cellular Adaptation to Hyperosmotic Stress in Arabidopsis

doi:10.3390/ijms222212534

	Both Clathrin-Mediated and Membrane Microdomain-Associated Endocytosis Contribute to the Cellular Adaptation to Hyperosmotic Stress in Arabidopsis
	Wu, Zheng 1,2; Fan, Chengyu3 ; Man, Yi 1,2; Zhang, Yue 1,2; Li, Ruili 1,2,4; Li, Xiaojuan 1,2,4; Jing, Yanping 1,2,4
	2021-11
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (IF:4.9[JCR-2023],5.6[5-Year])
EISSN	1422-0067
卷号	22 期号:22
DOI	10.3390/ijms222212534
摘要	As sessile organisms, plants must directly deal with an often complex and adverse environment in which hyperosmotic stress is one of the most serious abiotic factors, challenging cellular physiology and integrity. The plasma membrane (PM) is the hydrophobic barrier between the inside and outside environments of cells and is considered a central compartment in cellular adaptation to diverse stress conditions through dynamic PM remodeling. Endocytosis is a powerful method for rapid remodeling of the PM. In animal cells, different endocytic pathways are activated in response to osmotic stress, while only a few reports are related to the endocytosis response pathway and involve a mechanism in plant cells upon hyperosmotic stress. In this study, using different endocytosis inhibitors, the microdomain-specific dye di-4-ANEPPDHQ, variable-angle total internal reflection fluorescence microscopy (VA-TIRFM), and confocal microscopy, we discovered that internalized Clathrin Light Chain-Green Fluorescent Protein (CLC-GFP) increased under hyperosmotic conditions, accompanied by decreased fluorescence intensity of CLC-GFP at the PM. CLC-GFP tended to have higher diffusion coefficients and a fraction of CLC-GFP molecules underwent slower diffusion upon hyperosmotic stress. Meanwhile, an increased motion range of CLC-GFP was found under hyperosmotic treatment compared with the control. In addition, the order of the PM decreased, but the order of the endosome increased when cells were in hyperosmotic conditions. Hence, our results demonstrated that clathrin-mediated endocytosis and membrane microdomain-associated endocytosis both participate in the adaptation to hyperosmotic stress. These findings will help to further understand the role and the regulatory mechanism involved in plant endocytosis in helping plants adapt to osmotic stress.
关键词	endocytosis clathrin microdomain hyperosmotic stress Arabidopsis thaliana
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收录类别	SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000724944100001
出版者	MDPI
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/134148
专题	生命科学与技术学院_公共科研平台_分子影像平台
通讯作者	Jing, Yanping
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, 35 Qinghua East Rd, Beijing 100083, Peoples R China; 2.Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, 35 Qinghua East Rd, Beijing 100083, Peoples R China; 3.Shanghai Tech Univ, Sch Life Sci & Technol, Mol Imaging Core Facil, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China; 4.Beijing Forestry Univ, Tree & Ornamental Plant Breeding & Biotechnol Lab, 35 Qinghua East Rd, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Wu, Zheng,Fan, Chengyu,Man, Yi,et al. Both Clathrin-Mediated and Membrane Microdomain-Associated Endocytosis Contribute to the Cellular Adaptation to Hyperosmotic Stress in Arabidopsis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(22).
APA	Wu, Zheng.,Fan, Chengyu.,Man, Yi.,Zhang, Yue.,Li, Ruili.,...&Jing, Yanping.(2021).Both Clathrin-Mediated and Membrane Microdomain-Associated Endocytosis Contribute to the Cellular Adaptation to Hyperosmotic Stress in Arabidopsis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(22).
MLA	Wu, Zheng,et al."Both Clathrin-Mediated and Membrane Microdomain-Associated Endocytosis Contribute to the Cellular Adaptation to Hyperosmotic Stress in Arabidopsis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.22(2021).