Designing Modular Cell-free Systems for Tunable Biotransformation of L-phenylalanine to Aromatic Compounds

doi:10.3389/fbioe.2021.730663

	Designing Modular Cell-free Systems for Tunable Biotransformation of L-phenylalanine to Aromatic Compounds
	Yang, Chen1,2,3 ; Liu, Yushi1 ; Liu, Wan-Qiu1 ; Wu, Changzhu 4,5; Li, Jian1
	2021-07-28
发表期刊	FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (IF:4.3[JCR-2023],5.1[5-Year])
ISSN	2296-4185
EISSN	22964185
卷号	9
DOI	10.3389/fbioe.2021.730663
摘要	Cell-free systems have been used to synthesize chemicals by reconstitution of in vitro expressed enzymes. However, coexpression of multiple enzymes to reconstitute long enzymatic pathways is often problematic due to resource limitation/competition (e.g., energy) in the one-pot cell-free reactions. To address this limitation, here we aim to design a modular, cell-free platform to construct long biosynthetic pathways for tunable synthesis of value-added aromatic compounds, using (S)-1-phenyl-1,2-ethanediol ((S)-PED) and 2-phenylethanol (2-PE) as models. Initially, all enzymes involved in the biosynthetic pathways were individually expressed by an E. coli-based cell-free protein synthesis (CFPS) system and their catalytic activities were confirmed. Then, three sets of enzymes were coexpressed in three cell-free modules and each with the ability to complete a partial pathway. Finally, the full biosynthetic pathways were reconstituted by mixing two related modules to synthesize (S)-PED and 2-PE, respectively. After optimization, the final conversion rates for (S)-PED and 2-PE reached 100 and 82.5%, respectively, based on the starting substrate of L-phenylalanine. We anticipate that the modular cell-free approach will make a possible efficient and high-yielding biosynthesis of value-added chemicals.
关键词	cell-free systems biocatalysis biotransformation value-added chemicals synthetic biology Amino acids Aromatic compounds Biochemistry Bioconversion Biosynthesis Catalyst activity Cells Enzymes Escherichia coli Biosynthetic pathway Cell free protein synthesis Cell free reactions free system Conversion rates Enzymatic pathways Resource limitations Value added chemicals
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收录类别	SCIE ; EI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS类目	Biotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS记录号	WOS:000683849200001
出版者	FRONTIERS MEDIA SA
EI入藏号	20213310766735
EI主题词	Cytology
EI分类号	461.2 Biological Materials and Tissue Engineering ; 801.2 Biochemistry ; 803 Chemical Agents and Basic Industrial Chemicals ; 804 Chemical Products Generally ; 804.1 Organic Compounds
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127936
专题	物质科学与技术学院_PI研究组_李健组物质科学与技术学院_硕士生
通讯作者	Li, Jian
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.Univ Southern Denmark, Danish Inst Adv Study DIAS, Odense, Denmark; 5.Univ Southern Denmark, Dept Phys Chem & Pharm, Odense, Denmark
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Yang, Chen,Liu, Yushi,Liu, Wan-Qiu,et al. Designing Modular Cell-free Systems for Tunable Biotransformation of L-phenylalanine to Aromatic Compounds[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2021,9.
APA	Yang, Chen,Liu, Yushi,Liu, Wan-Qiu,Wu, Changzhu,&Li, Jian.(2021).Designing Modular Cell-free Systems for Tunable Biotransformation of L-phenylalanine to Aromatic Compounds.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,9.
MLA	Yang, Chen,et al."Designing Modular Cell-free Systems for Tunable Biotransformation of L-phenylalanine to Aromatic Compounds".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 9(2021).