High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics

doi:10.1126/sciadv.abe3839

	High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics
	Wang, Yuan 1,2; Jin, Ruina 1,2; Shen, Bingqing 3; Li, Na 1,2; Zhou, He 3; Wang, Wei4 ; Zhao, Yingjie 5,6; Huang, Mengshi 3; Fang, Pan 3; Wang, Shanshan 3; Mary, Pascaline 3; Wang, Ruikun 1,2; Ma, Peixiang4 ; Li, Ruonan 1,2; Tian, Yujie 1,2; Cao, Youjia 1,2; Li, Fubin 5,6; Schweizer, Liang 3; Zhang, Hongkai1,2,4
	2021-06
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	7 期号:24
DOI	10.1126/sciadv.abe3839
摘要	Currently, high-throughput approaches are lacking in the isolation of antibodies with functional readouts beyond simple binding. This situation has impeded the next generation of cancer immunotherapeutics, such as bispecific T cell engager (BiTE) antibodies or agonist antibodies against costimulatory receptors, from reaching their full potential. Here, we developed a highly efficient droplet-based microfluidic platform combining a lentivirus transduction system that enables functional screening of millions of antibodies to identify potential hits with desired functionalities. To showcase the capacity of this system, functional antibodies for CD40 agonism with low frequency (<0.02%) were identified with two rounds of screening. Furthermore, the versatility of the system was demonstrated by combining an anti-Her2 x anti-CD3 BiTE antibody library with functional screening, which enabled efficient identification of active anti-Her2 x anti-CD3 BiTE antibodies. The platform could revolutionize next-generation cancer immunotherapy drug development and advance medical research.
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收录类别	SCIE ; EI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000677572900002
出版者	AMER ASSOC ADVANCEMENT SCIENCE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127872
专题	免疫化学研究所_特聘教授组_张宏恺组免疫化学研究所_特聘教授组_功能筛选实验室免疫化学研究所_公共科研平台_生物医学大数据平台
通讯作者	Zhang, Hongkai
作者单位	1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin, Peoples R China; 2.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China; 3.HiFiBiO Shanghai Co Ltd, Shanghai, Peoples R China; 4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China; 5.Shanghai Jiao Tong Univ, Sch Med, Fac Basic Med, Shanghai Inst Immunol, Shanghai, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Wang, Yuan,Jin, Ruina,Shen, Bingqing,et al. High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics[J]. SCIENCE ADVANCES,2021,7(24).
APA	Wang, Yuan.,Jin, Ruina.,Shen, Bingqing.,Li, Na.,Zhou, He.,...&Zhang, Hongkai.(2021).High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics.SCIENCE ADVANCES,7(24).
MLA	Wang, Yuan,et al."High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics".SCIENCE ADVANCES 7.24(2021).