ShanghaiTech University Knowledge Management System
| High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics | |
| 2021-06 | |
| Source Publication | SCIENCE ADVANCES
 |
| ISSN | 2375-2548 |
| Volume | 7Issue:24 |
| DOI | 10.1126/sciadv.abe3839 |
| Abstract | Currently, high-throughput approaches are lacking in the isolation of antibodies with functional readouts beyond simple binding. This situation has impeded the next generation of cancer immunotherapeutics, such as bispecific T cell engager (BiTE) antibodies or agonist antibodies against costimulatory receptors, from reaching their full potential. Here, we developed a highly efficient droplet-based microfluidic platform combining a lentivirus transduction system that enables functional screening of millions of antibodies to identify potential hits with desired functionalities. To showcase the capacity of this system, functional antibodies for CD40 agonism with low frequency (<0.02%) were identified with two rounds of screening. Furthermore, the versatility of the system was demonstrated by combining an anti-Her2 x anti-CD3 BiTE antibody library with functional screening, which enabled efficient identification of active anti-Her2 x anti-CD3 BiTE antibodies. The platform could revolutionize next-generation cancer immunotherapy drug development and advance medical research. |
| URL | 查看原文 |
| Indexed By | SCIE ; EI |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000677572900002 |
| Publisher | AMER ASSOC ADVANCEMENT SCIENCE |
| Original Document Type | Article |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127872 |
| Collection | 免疫化学研究所_特聘教授组_张宏恺组 免疫化学研究所_特聘教授组_功能筛选实验室 免疫化学研究所_公共科研平台_生物医学大数据平台 |
| Corresponding Author | Zhang, Hongkai |
| Affiliation | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin, Peoples R China; 2.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China; 3.HiFiBiO Shanghai Co Ltd, Shanghai, Peoples R China; 4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China; 5.Shanghai Jiao Tong Univ, Sch Med, Fac Basic Med, Shanghai Inst Immunol, Shanghai, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai, Peoples R China |
| Corresponding Author Affilication | Shanghai Institute for Advanced Immunochemical Studies |
| Recommended Citation GB/T 7714 | Wang, Yuan,Jin, Ruina,Shen, Bingqing,et al. High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics[J]. SCIENCE ADVANCES,2021,7(24). |
| APA | Wang, Yuan.,Jin, Ruina.,Shen, Bingqing.,Li, Na.,Zhou, He.,...&Zhang, Hongkai.(2021).High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics.SCIENCE ADVANCES,7(24). |
| MLA | Wang, Yuan,et al."High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics".SCIENCE ADVANCES 7.24(2021). |
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