High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics
2021-06
Source PublicationSCIENCE ADVANCES
ISSN2375-2548
Volume7Issue:24
DOI10.1126/sciadv.abe3839
AbstractCurrently, high-throughput approaches are lacking in the isolation of antibodies with functional readouts beyond simple binding. This situation has impeded the next generation of cancer immunotherapeutics, such as bispecific T cell engager (BiTE) antibodies or agonist antibodies against costimulatory receptors, from reaching their full potential. Here, we developed a highly efficient droplet-based microfluidic platform combining a lentivirus transduction system that enables functional screening of millions of antibodies to identify potential hits with desired functionalities. To showcase the capacity of this system, functional antibodies for CD40 agonism with low frequency (<0.02%) were identified with two rounds of screening. Furthermore, the versatility of the system was demonstrated by combining an anti-Her2 x anti-CD3 BiTE antibody library with functional screening, which enabled efficient identification of active anti-Her2 x anti-CD3 BiTE antibodies. The platform could revolutionize next-generation cancer immunotherapy drug development and advance medical research.
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Indexed BySCIE ; EI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000677572900002
PublisherAMER ASSOC ADVANCEMENT SCIENCE
Original Document TypeArticle
Citation statistics
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127872
Collection免疫化学研究所_特聘教授组_张宏恺组
免疫化学研究所_特聘教授组_功能筛选实验室
免疫化学研究所_公共科研平台_生物医学大数据平台
Corresponding AuthorZhang, Hongkai
Affiliation
1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin, Peoples R China;
2.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China;
3.HiFiBiO Shanghai Co Ltd, Shanghai, Peoples R China;
4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China;
5.Shanghai Jiao Tong Univ, Sch Med, Fac Basic Med, Shanghai Inst Immunol, Shanghai, Peoples R China;
6.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai, Peoples R China
Corresponding Author AffilicationShanghai Institute for Advanced Immunochemical Studies
Recommended Citation
GB/T 7714
Wang, Yuan,Jin, Ruina,Shen, Bingqing,et al. High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics[J]. SCIENCE ADVANCES,2021,7(24).
APA Wang, Yuan.,Jin, Ruina.,Shen, Bingqing.,Li, Na.,Zhou, He.,...&Zhang, Hongkai.(2021).High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics.SCIENCE ADVANCES,7(24).
MLA Wang, Yuan,et al."High-throughput functional screening for next-generation cancer immunotherapy using droplet-based microfluidics".SCIENCE ADVANCES 7.24(2021).
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