RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis

doi:10.1111/cpr.13098

	RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis
	Yang, Chao 1; Yao, Chencheng 1; Ji, Zhiyong 2; Zhao, Liangyu 1; Chen, Huixing 1; Li, Peng 1; Tian, Ruhui 1; Zhi, Erlei 1; Huang, Yuhua 1; Han, Xia 1; Hong, Yan 1; Zhou, Zhi3 ; Li, Zheng 1,2
	2021-09
发表期刊	CELL PROLIFERATION (IF:5.9[JCR-2023],7.3[5-Year])
ISSN	0960-7722
EISSN	1365-2184
卷号	54 期号:9
DOI	10.1111/cpr.13098
摘要	Objectives RNA-binding proteins (RBPs) play essential post-transcriptional roles in regulating spermatogonial stem cells (SSCs) maintenance and differentiation. We identified a conserved and SSCs-enriched RBP ELAVL2 from our single-cell sequencing data, but its function and mechanism in SSCs were unclear. Materials and methods Expressions of ELAVL2 during human and mouse testis development were validated. Stable C18-4 and TCam-2 cell lines with overexpression and knockdown of ELAVL2 were established, which were applied to proliferation and apoptosis analysis. RNA immunoprecipitation and sequencing were used to identify ELAVL2 targets, and regulatory functions of ELAVL2 on target mRNAs were studied. Proteins interacting with ELAVL2 in human and mouse testes were identified using immunoprecipitation and mass spectrometric, which were validated by in vivo and in vitro experiments. Results ELAVL2 was testis-enriched and preferentially expressed in human and mouse SSCs. ELAVL2 was down-regulated in NOA patients. ELAVL2 promoted proliferation and inhibited apoptosis of C18-4 and TCam-2 cell lines via activating ERK and AKT pathways. ELAVL2 associated with mRNAs encoding essential regulators of SSCs proliferation and survival, and promoted their protein expression at post-transcriptional level. ELAVL2 interacted with DAZL in vivo and in vitro in both human and mouse testes. Conclusions Taken together, these results indicate that ELAVL2 is a conserved SSCs-enriched RBP that down-regulated in NOA, which regulates spermatogonia proliferation and apoptosis by promoting protein expression of targets.
关键词	azoospermia male infertility post-transcriptional regulation RNA-binding protein spermatogonial stem cells
收录类别	SCIE
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000676096400001
出版者	WILEY
原始文献类型	Article; Early Access
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127851
专题	生命科学与技术学院_PI研究组_周智组
通讯作者	Zhou, Zhi; Li, Zheng
作者单位	1.Shanghai Jiao Tong Univ, Shanghai Gen Hosp, Urol Med Ctr, Dept Androl,Ctr Mens Hlth,Sch Med, Shanghai, Peoples R China; 2.Nanjing Med Univ, State Key Lab Reprod Med, Nanjing, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Yang, Chao,Yao, Chencheng,Ji, Zhiyong,et al. RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis[J]. CELL PROLIFERATION,2021,54(9).
APA	Yang, Chao.,Yao, Chencheng.,Ji, Zhiyong.,Zhao, Liangyu.,Chen, Huixing.,...&Li, Zheng.(2021).RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis.CELL PROLIFERATION,54(9).
MLA	Yang, Chao,et al."RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis".CELL PROLIFERATION 54.9(2021).