Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum

doi:10.3389/fmicb.2021.692957

	Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum
	Gao, Yuqi 1; Niu, Mingze 1; Yu, Xiaohui 1; Bao, Tingting 1; Wu, Zhaowei2 ; Zhao, Xin 1,3
	2021-06-21
发表期刊	FRONTIERS IN MICROBIOLOGY (IF:4.0[JCR-2023],5.1[5-Year])
EISSN	1664-302X
卷号	12
DOI	10.3389/fmicb.2021.692957
摘要	Lactiplantibacillus plantarum are probiotic bacteria, maintaining the integrity of the gastrointestinal epithelial barrier, and preventing the infection of pathogenic bacteria. Exopolysaccharides (EPSs) are often involved in the probiotic property of L. plantarum. Here, we identified a new EPS-synthetic gene cluster, cpsWc, carrying 13 genes, laid on a large plasmid in a well-characterized probiotic L. plantarum strain LTC-113. The cpsWc gene cluster was horizontally acquired from Weissella cibaria, enhancing the biofilm formation ability of the host strain and its tolerance to harsh environmental stresses, including heat, acid, and bile. Transfer of cpsWc also conferred the probiotic properties to other L. plantarum strains. Moreover, cpsWc strengthened the adhesion of LTC-113 to intestinal epithelial cells. Both the cpsWc-carrying LTC-113 and its EPSs per se effectively attenuated the LPS-induced pro-inflammatory effect of intestinal epithelial cells, and inhibited the adhesion of pathogenic bacteria, such as S. typhimurium and E. coli by exclusion and competition. The newly identified cpsWc gene cluster emphasized the contribution of mobile EPS-synthetic element on the probiotic activity of L. plantarum, and shed a light on the engineering of probiotic bacteria.
关键词	Lactiplantibacillus plantarum cpsWc exopolysaccharides biofilm adhesion
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收录类别	SCIE
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000669615900001
出版者	FRONTIERS MEDIA SA
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127737
专题	物质科学与技术学院_PI研究组_季泉江组
通讯作者	Zhao, Xin
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China; 2.Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai, Peoples R China; 3.McGill Univ, Dept Anim Sci, Montreal, PQ, Canada
推荐引用方式 GB/T 7714	Gao, Yuqi,Niu, Mingze,Yu, Xiaohui,et al. Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum[J]. FRONTIERS IN MICROBIOLOGY,2021,12.
APA	Gao, Yuqi,Niu, Mingze,Yu, Xiaohui,Bao, Tingting,Wu, Zhaowei,&Zhao, Xin.(2021).Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum.FRONTIERS IN MICROBIOLOGY,12.
MLA	Gao, Yuqi,et al."Horizontally Acquired Polysaccharide-Synthetic Gene Cluster From Weissella cibaria Boosts the Probiotic Property of Lactiplantibacillus plantarum".FRONTIERS IN MICROBIOLOGY 12(2021).