上海科技大学知识管理系统

本发明公开了一种双荧光发射峰调控的下转换纳米材料及其制备方法与应用。所述下转换纳米材料具有核壳结构，由内向外依次包括作为核层的Er发射层及作为壳层的隔绝层、Nd发射层和钝化层，所述下转换纳米材料的双荧光发射峰分别为1055～1065nm和1520～1535nm。本发明合成下转换纳米材料的条件可控性好，重复性高；所获下转换纳米材料的粒径均一，高度分散；通过聚合物包覆的方法对其表面进行功能化处理，再利用静电相互作用负载荧光淬灭剂，制得激活型的比率型探针，可实现高的组织穿透深度和高时空分辨率；同时能够特异性检测生物体内类风湿性关节炎的炎症因子ONOO‑。

The invention discloses a down-conversion nano material controlled by double fluorescent emission peaks as well as a preparation method and application thereof. The down-conversion nano-material has a core-shell structure, Er emitting layers as a core layer and an insulating layer serving as shell layers are sequentially arranged from inside to outside. Nd Emission layer and passivation layer, the double fluorescence emission peak of down conversion nano material is 1055 - 1065 nm and 1520 - 1535 nm . and the condition controllability is good, and the repeatability is high for synthesizing down conversion nanometer material respectively. The obtained down-conversion nano-material is uniform in particle size and high in dispersity. The surface of the method is functionalized and treated by the polymer coating method, and then the electrostatic interaction load fluorescence quenching agent is utilized to prepare an activated ratio type probe, so that high tissue penetration depth and high space-time resolution can be achieved. Inflammation factor ONOO capable of specifically detecting rheumatoid arthritis in vivo. .