Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

doi:10.1038/s41467-021-22352-4

	Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root
	Zhang, Tian-Qi 1; Chen, Yu 1,2; Liu, Ye 3; Lin, Wen-Hui 4; Wang, Jia-Wei1,5
	2021-04-06
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
ISSN	2041-1723
卷号	12 期号:1
DOI	10.1038/s41467-021-22352-4
摘要	Root development relies on the establishment of meristematic tissues that give rise to distinct cell types that differentiate across defined temporal and spatial gradients. Dissection of the developmental trajectories and the transcriptional networks that underlie them could aid understanding of the function of the root apical meristem in both dicots and monocots. Here, we present a single-cell RNA (scRNA) sequencing and chromatin accessibility survey of rice radicles. By temporal profiling of individual root tip cells we reconstruct continuous developmental trajectories of epidermal cells and ground tissues, and elucidate regulatory networks underlying cell fate determination in these cell lineages. We further identify characteristic processes, transcriptome profiles, and marker genes for these cell types and reveal conserved and divergent root developmental pathways between dicots and monocots. Finally, we demonstrate the potential of the platform for functional genetic studies by using spatiotemporal modeling to identify a rice root meristematic mutant from a cell-specific gene cohort. Root meristems give rise to distinct cell types that differentiate across defined temporal and spatial gradients. Here, via single-cell RNA sequencing and surveying chromatin accessibility the authors profile gene expression of different cell types during rice root development.
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收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000661132800013
出版者	NATURE RESEARCH
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127583
专题	生命科学与技术学院_特聘教授组_王佳伟组
通讯作者	Zhang, Tian-Qi; Wang, Jia-Wei
作者单位	1.Chinese Acad Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Shanghai, Peoples R China; 3.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Lab Landscap,Minist Agr & Rural Affairs, Nanjing, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Joint Ctr Single Cell Biol, Shanghai, Peoples R China; 5.Shanghai Tech Univ, Shanghai 200031, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Zhang, Tian-Qi,Chen, Yu,Liu, Ye,et al. Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root[J]. NATURE COMMUNICATIONS,2021,12(1).
APA	Zhang, Tian-Qi,Chen, Yu,Liu, Ye,Lin, Wen-Hui,&Wang, Jia-Wei.(2021).Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root.NATURE COMMUNICATIONS,12(1).
MLA	Zhang, Tian-Qi,et al."Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root".NATURE COMMUNICATIONS 12.1(2021).