General method to stabilize mesophilic proteins in hyperthermal water

doi:10.1016/j.isci.2021.102503

	General method to stabilize mesophilic proteins in hyperthermal water
	Xin, Xiaoqian 1; Xu, Youwei2 ; Shi, Hu 3; Liu, Xiaowen 1
	2021-05-21
发表期刊	ISCIENCE (IF:4.6[JCR-2023],5.0[5-Year])
EISSN	2589-0042
卷号	24 期号:5
DOI	10.1016/j.isci.2021.102503
摘要	The stability of protein structures and biological functions at normal temperature is closely linked with the universal aqueous environment of organisms. Preserving bioactivities of proteins in hyperthermia water would expand their functional capabilities beyond those in native environments. However, only a limited number of proteins derived from hyperthermophiles are thermostable at elevated temperatures. Triggered by this, here we describe a general method to stabilize mesophilic proteins in hyperthermia water. The mesophilic proteins, protected by amphiphilic polymers with multiple binding sites, maintain their secondary and tertiary structures after incubation even in boiling water. This approach, outside the conventional environment for bioactivities of mesophilic proteins, provides a general strategy to dramatically increase the T-m (melting temperature) of mesophilic proteins without any changes to amino sequences of the native proteins. Current work offers a new insight with protein stability engineering for potential application, including vaccine storage and enzyme engineering.
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000653990500107
出版者	CELL PRESS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/127538
专题	免疫化学研究所_PI研究组_杨贝组
通讯作者	Liu, Xiaowen
作者单位	1.Jinan Univ, Clin Translat Ctr Targeted Drug, Sch Med, Guangzhou 510632, Peoples R China; 2.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China; 3.Shanxi Univ, Sch Chem & Chem Engn, Inst Mol Sci, Taiyuan 030006, Peoples R China
推荐引用方式 GB/T 7714	Xin, Xiaoqian,Xu, Youwei,Shi, Hu,et al. General method to stabilize mesophilic proteins in hyperthermal water[J]. ISCIENCE,2021,24(5).
APA	Xin, Xiaoqian,Xu, Youwei,Shi, Hu,&Liu, Xiaowen.(2021).General method to stabilize mesophilic proteins in hyperthermal water.ISCIENCE,24(5).
MLA	Xin, Xiaoqian,et al."General method to stabilize mesophilic proteins in hyperthermal water".ISCIENCE 24.5(2021).