上海科技大学知识管理系统

Compositions and methods for detecting molecule-molecule interactions are provided. The methods employ a prokaryotic ubiquitin-like protein (Pup) and a Pup ligase that is coupled to one of the molecules. When the Pup ligase is brought to proximity to the other molecule by virtue of the molecule-molecule interaction, the Pup ligase can conjugate the Pup to a lysine residue on the other molecule. As such conjugation can be easily detected, this method allows easy identification of the molecule-molecule interaction.

本发明提供了用于检测分子-分子相互作用的组合物和方法。 所述方法使用原核泛素样蛋白(PUP)和偶联到所述分子之一的PUP连接酶。 当通过分子-分子相互作用使PUP连接酶接近另一分子时，PUP连接酶可将PUP偶联到另一分子上的赖氨酸残基上。 因为这种偶联可以容易地检测，所以该方法允许容易地鉴定分子-分子相互作用。