SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production

doi:10.1016/j.scib.2021.01.013

	SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production
	Luo, Lingling1,2 ; Li, Zhean2,3 ; Zhao, Tiejun 4; Ju, Xiaohui 5; Ma, Peixiang3 ; Jin, Boxing 4; Zhou, Yulin 4; He, Su 4; Huang, Jinhua 4; Xu, Xun 5; Zou, Yan3 ; Li, Ping 6; Liang, Aibin 6; Liu, Jia3 ; Chi, Tian2 ; Huang, Xingxu2 ; Ding, Qiang 5,7; Jin, Zhigang 4; Huang, Cheng 1; Zhang, Yu2
	2021-06-30
发表期刊	SCIENCE BULLETIN (IF:18.8[JCR-2023],15.8[5-Year])
ISSN	2095-9273
EISSN	2095-9281
卷号	66 期号:12 页码:1194-1204
DOI	10.1016/j.scib.2021.01.013
摘要	A key to tackling the coronavirus disease 2019 (COVID-19) pandemic is to understand how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) manages to outsmart host antiviral defense mechanisms. Stress granules (SGs), which are assembled during viral infection and function to sequester host and viral mRNAs and proteins, are part of the antiviral responses. Here, we show that the SARS-CoV-2 nucleocapsid (N) protein, an RNA binding protein essential for viral production, interacted with RasGTPase-activating protein SH3-domain-binding protein (G3BP) and disrupted SG assembly, both of which require intrinsically disordered region1 (IDR1) in N protein. The N protein partitioned into SGs through liquid-liquid phase separation with G3BP, and blocked the interaction of G3BP1 with other SG-related proteins. Moreover, the N protein domains important for phase separation with G3BP and SG disassembly were required for SARS-CoV-2 viral production. We propose that N protein-mediated SG disassembly is crucial for SARS-CoV-2 production. (c) 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.
关键词	SARS-CoV-2 Nucleocapsid protein Stress granules Liquid-liquid phase separation G3BP Viral production
收录类别	SCIE ; EI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000657605600009
出版者	ELSEVIER
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126988
专题	生命科学与技术学院_PI研究组_黄行许组生命科学与技术学院_PI研究组_池天组免疫化学研究所_特聘教授组_功能筛选实验室免疫化学研究所_PI研究组_刘佳组
通讯作者	Huang, Xingxu; Ding, Qiang; Jin, Zhigang; Huang, Cheng; Zhang, Yu
作者单位	1.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China; 4.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China; 5.Tsinghua Univ, Ctr Infect Dis Res, Sch Med, Beijing 100084, Peoples R China; 6.Tongji Univ, Dept Hematol, Tongji Hosp, Shanghai 200065, Peoples R China; 7.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Beijing 100084, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Luo, Lingling,Li, Zhean,Zhao, Tiejun,et al. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production[J]. SCIENCE BULLETIN,2021,66(12):1194-1204.
APA	Luo, Lingling.,Li, Zhean.,Zhao, Tiejun.,Ju, Xiaohui.,Ma, Peixiang.,...&Zhang, Yu.(2021).SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production.SCIENCE BULLETIN,66(12),1194-1204.
MLA	Luo, Lingling,et al."SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production".SCIENCE BULLETIN 66.12(2021):1194-1204.