ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae

doi:10.1093/g3journal/jkaa060

	ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae
	Zhang, Shanshan1,2,3 ; Feng, Han-Chao1 ; Liu, Ji-Long1,4
	2021-01
发表期刊	G3-GENES GENOMES GENETICS (IF:2.1[JCR-2023],2.5[5-Year])
ISSN	2160-1836
卷号	11 期号:1
DOI	10.1093/g3journal/jkaa060
摘要	Asparagine synthetase (ASNS) and CTP synthase (CTPS) are two metabolic enzymes that catalyze the biosynthesis of asparagine and CTP, respectively. Both CTPS and ASNS have been identified to form cytoophidia in Saccharomyces cerevisiae. Glutamine is a common substrate for both these enzymes, and they play an important role in glutamine homeostasis. Here, we find that the ASNS cytoophidia are shorter than the CTPS cytoophidia, and that disruption of ASNS shortens the length of CTPS cytoophidia. However, the deletion of CTPS has no effect on the formation and length of ASNS cytoophidia, or on the ASNS protein level. We also find that Asn1 overexpression induces the formation of a multi-dot structure in diauxic phase which suggests that the increased protein level may trigger cytoophidia formation. Collectively, our results reveal a connection between ASNS cytoophidia and CTPS cytoophidia.
关键词	asparagine synthetase CTP synthase cytoophidium glutamine metabolism Saccharomyces cerevisiae
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收录类别	SCIE
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000651851700033
出版者	OXFORD UNIV PRESS INC
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126816
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_刘冀珑组生命科学与技术学院_本科生
通讯作者	Liu, Ji-Long
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; 4.Univ Oxford, Dept Physiol, Oxford OX1 3PT, England
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Shanshan,Feng, Han-Chao,Liu, Ji-Long. ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae[J]. G3-GENES GENOMES GENETICS,2021,11(1).
APA	Zhang, Shanshan,Feng, Han-Chao,&Liu, Ji-Long.(2021).ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae.G3-GENES GENOMES GENETICS,11(1).
MLA	Zhang, Shanshan,et al."ASNS disruption shortens CTPS cytoophidia in Saccharomyces cerevisiae".G3-GENES GENOMES GENETICS 11.1(2021).