Proteomic Exploration of Endocytosis of Framework Nucleic Acids

doi:10.1002/smll.202100837

	Proteomic Exploration of Endocytosis of Framework Nucleic Acids
	Tian, Tian 1; Zhang, Chengqian2 ; Li, Jiang 3; Liu, Yifan 1; Wang, Yue 1; Ke, Xisong 1; Fan, Chunhai 4,5; Lei, Haozhi 6; Hao, Piliang2 ; Li, Qian 4,5
	2021-06
发表期刊	SMALL (IF:13.0[JCR-2023],13.5[5-Year])
ISSN	1613-6810
EISSN	1613-6829
卷号	17 期号:23
DOI	10.1002/smll.202100837
摘要	Efficient cell internalization of framework nucleic acid nanostructures free of transfection agents provides new opportunities for developing biocompatible and intelligent nanoprobes and drug delivery carriers. Here, a proteomic identification method to screen target proteins that interact with tetrahedral DNA nanostructures (TDNs) during the process of endocytosis by combining drug affinity responsive target stability (DARTS) with liquid chromatography/tandem mass spectrometry (LC-MS/MS) techniques, is reported. It is found that that caveolin-1 (CAV1) and macropinocytosis-related protein sorting nexin5 (SNX5) are associated with the endocytosis of TNDs, which is further validated by microscale thermophoresis (MST) analysis. CAV1- and SNX5- knockout experiments reveal that both caveolae-mediated endocytosis and macropinocytosis mediate the cellular uptake of TDNs, which complement previous findings with fluorescence tracing methods. This method provides a generic strategy to analyze cellular internalization process of DNA nanostructures for biomedical applications.
关键词	caveolin DNA nanostructures endocytosis macropinocytosis proteomic exploration
收录类别	SCIE ; EI
语种	英语
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS记录号	WOS:000643153700001
出版者	WILEY-V C H VERLAG GMBH
原始文献类型	Article; Early Access
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126688
专题	生命科学与技术学院_公共科研平台_组学分析平台
通讯作者	Lei, Haozhi; Hao, Piliang; Li, Qian
作者单位	1.Shanghai Univ Tradit Chinese Med, Ctr Chem Biol, Inst Interdisciplinary Integrat Med Res, Shanghai 201203, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, Shanghai Adv Res Inst, Zhangjiang Lab,Bioimaging Ctr, Shanghai 201210, Peoples R China; 4.Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China; 5.Shanghai Jiao Tong Univ, Natl Ctr Translat Med, Shanghai 200240, Peoples R China; 6.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200127, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Tian, Tian,Zhang, Chengqian,Li, Jiang,et al. Proteomic Exploration of Endocytosis of Framework Nucleic Acids[J]. SMALL,2021,17(23).
APA	Tian, Tian.,Zhang, Chengqian.,Li, Jiang.,Liu, Yifan.,Wang, Yue.,...&Li, Qian.(2021).Proteomic Exploration of Endocytosis of Framework Nucleic Acids.SMALL,17(23).
MLA	Tian, Tian,et al."Proteomic Exploration of Endocytosis of Framework Nucleic Acids".SMALL 17.23(2021).