Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm
Zhang, Xiaoning1,2; Zhou, Wenwen1; Zhang, Peng2; Gao, Fengxin2; Zhao, Xiuling1; Shum, Winnie Waichi3; Zeng, Xuhui1,2
2021-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN1422-0067
Volume22Issue:2Pages:#VALUE!
DOI10.3390/ijms22020652
AbstractThe calcium-binding protein spermatid-associated 1 (Cabs1) is a novel spermatid-specific protein. However, its function remains largely unknown. In this study, we found that a long noncoding RNA (lncRNA) transcripted from the Cabs1 gene antisense, AntiCabs1, was also exclusively expressed in spermatids. Cabs1 and AntiCabs1 knockout mice were generated separately (using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 methods) to investigate their functions in spermatogenesis. The genetic loss of Cabs1 did not affect testicular and epididymal development; however, male mice exhibited significantly impaired sperm tail structure and subfertility. Ultrastructural analysis revealed defects in sperm flagellar differentiation leading to an abnormal annulus and disorganization of the midpiece-principal piece junction, which may explain the high proportion of sperm with a bent tail. Interestingly, the proportion of sperm with a bent tail increased during transit in the epididymis. Furthermore, Western blot and immunofluorescence analyses showed that a genetic loss of Cabs1 decreased Septin 4 and Krt1 and increased cyclin Y-like 1 (Ccnyl1) levels compared with the wild type, suggesting that Cabs1 deficiency disturbed the expression of cytoskeleton-related proteins. By contrast, AntiCabs1(-/-) mice were indistinguishable from the wild type regarding testicular and epididymal development, sperm morphology, concentration and motility, and male fertility. This study demonstrates that Cabs1 is an important component of the sperm annulus essential for proper sperm tail assembly and motility.
KeywordCabs1 AntiCabs1 lncRNA spermiogenesis spermatogenesis
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000611332400001
PublisherMDPI
WOS KeywordCALCIUM-BINDING PROTEIN ; LONG NONCODING RNAS ; TERMINAL DIFFERENTIATION ; MALE-FERTILITY ; GERM-CELLS ; EXPRESSION ; SPERMATOGENESIS ; SPERMIOGENESIS ; DELETION ; LACKING
Original Document TypeArticle
Citation statistics
Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125917
Collection生命科学与技术学院_PI研究组_岑慧枝组
Affiliation1.Nantong Univ, Inst Reprod Med, Med Sch, Nantong 226019, Peoples R China;
2.Nanchang Univ, Inst Life Sci, Nanchang 330031, Jiangxi, Peoples R China;
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xiaoning,Zhou, Wenwen,Zhang, Peng,et al. Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(2):#VALUE!.
APA Zhang, Xiaoning.,Zhou, Wenwen.,Zhang, Peng.,Gao, Fengxin.,Zhao, Xiuling.,...&Zeng, Xuhui.(2021).Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(2),#VALUE!.
MLA Zhang, Xiaoning,et al."Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.2(2021):#VALUE!.
Files in This Item: Download All
File Name/Size DocType Version Access License
10.3390@ijms22020652(34070KB)期刊论文出版稿开放获取CC BYView Download
Related Services
Usage statistics
Scholar Google
Similar articles in Scholar Google
[Zhang, Xiaoning]'s Articles
[Zhou, Wenwen]'s Articles
[Zhang, Peng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Xiaoning]'s Articles
[Zhou, Wenwen]'s Articles
[Zhang, Peng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Xiaoning]'s Articles
[Zhou, Wenwen]'s Articles
[Zhang, Peng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 10.3390@ijms22020652.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.