Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm

doi:10.3390/ijms22020652

	Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm
	Zhang, Xiaoning 1,2; Zhou, Wenwen 1; Zhang, Peng 2; Gao, Fengxin 2; Zhao, Xiuling 1; Shum, Winnie Waichi3 ; Zeng, Xuhui 1,2
	2021-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
EISSN	1422-0067
卷号	22 期号:2 页码:#VALUE!
DOI	10.3390/ijms22020652
摘要	The calcium-binding protein spermatid-associated 1 (Cabs1) is a novel spermatid-specific protein. However, its function remains largely unknown. In this study, we found that a long noncoding RNA (lncRNA) transcripted from the Cabs1 gene antisense, AntiCabs1, was also exclusively expressed in spermatids. Cabs1 and AntiCabs1 knockout mice were generated separately (using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 methods) to investigate their functions in spermatogenesis. The genetic loss of Cabs1 did not affect testicular and epididymal development; however, male mice exhibited significantly impaired sperm tail structure and subfertility. Ultrastructural analysis revealed defects in sperm flagellar differentiation leading to an abnormal annulus and disorganization of the midpiece-principal piece junction, which may explain the high proportion of sperm with a bent tail. Interestingly, the proportion of sperm with a bent tail increased during transit in the epididymis. Furthermore, Western blot and immunofluorescence analyses showed that a genetic loss of Cabs1 decreased Septin 4 and Krt1 and increased cyclin Y-like 1 (Ccnyl1) levels compared with the wild type, suggesting that Cabs1 deficiency disturbed the expression of cytoskeleton-related proteins. By contrast, AntiCabs1(-/-) mice were indistinguishable from the wild type regarding testicular and epididymal development, sperm morphology, concentration and motility, and male fertility. This study demonstrates that Cabs1 is an important component of the sperm annulus essential for proper sperm tail assembly and motility.
关键词	Cabs1 AntiCabs1 lncRNA spermiogenesis spermatogenesis
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收录类别	SCI ; SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000611332400001
出版者	MDPI
WOS关键词	CALCIUM-BINDING PROTEIN ; LONG NONCODING RNAS ; TERMINAL DIFFERENTIATION ; MALE-FERTILITY ; GERM-CELLS ; EXPRESSION ; SPERMATOGENESIS ; SPERMIOGENESIS ; DELETION ; LACKING
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125917
专题	生命科学与技术学院_PI研究组_岑慧枝组
通讯作者	Shum, Winnie Waichi; Zeng, Xuhui
作者单位	1.Nantong Univ, Inst Reprod Med, Med Sch, Nantong 226019, Peoples R China; 2.Nanchang Univ, Inst Life Sci, Nanchang 330031, Jiangxi, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Xiaoning,Zhou, Wenwen,Zhang, Peng,et al. Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(2):#VALUE!.
APA	Zhang, Xiaoning.,Zhou, Wenwen.,Zhang, Peng.,Gao, Fengxin.,Zhao, Xiuling.,...&Zeng, Xuhui.(2021).Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(2),#VALUE!.
MLA	Zhang, Xiaoning,et al."Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.2(2021):#VALUE!.