FGF13 Is Required for Histamine-Induced Itch Sensation by Interaction with Na(V)1.7

doi:10.1523/JNEUROSCI.0599-20.2020

	FGF13 Is Required for Histamine-Induced Itch Sensation by Interaction with Na(V)1.7
	Dong, Fei 1,2,4,5,6; Shi, Haixiang3,7 ; Yang, Liu 1,2; Xue, Huaqing 3; Wei, Manyi 3; Zhong, Yan-Qing 1,2,6; Bao, Lan3,7 ; Zhang, Xu1,2,4,5,6,7
	2020-12-09
发表期刊	JOURNAL OF NEUROSCIENCE (IF:4.4[JCR-2023],5.3[5-Year])
ISSN	0270-6474
卷号	40 期号:50 页码:9589-9601
发表状态	已发表
DOI	10.1523/JNEUROSCI.0599-20.2020
摘要	Itch can be induced by activation of small-diameter DRG neurons, which express abundant intracellular fibroblast growth factor 13 (FGF13). Although FGF13 is revealed to be essential for heat nociception, its role in mediating itch remains to be investigated. Here, we reported that loss of FGF13 in mouse DRG neurons impaired the histamine-induced scratching behavior. Calcium imaging showed that the percentage of histamine-responsive DRG neurons was largely decreased in FGF13-deficient mice; and consistently, electrophysiological recording exhibited that histamine failed to evoke action potential firing in most DRG neurons from these mice. Given that the reduced histamine-evoked neuronal response was caused by knockdown of FGF13 but not by FGF13A deficiency, FGF13B was supposed to mediate this process. Furthermore, overexpression of histamine Type 1 receptor H1R, but not H2R, H3R, nor H4R, increased the percentage of histamine-responsive DRG neurons, and the scratching behavior in FGF13-deficient mice was highly reduced by selective activation of H1R, suggesting that H1R is mainly required for FGF13-mediated neuronal response and scratching behavior induced by histamine. However, overexpression of H1R failed to rescue the histamine-evoked neuronal response in FGF13-deficient mice. Histamine enhanced the FGF13 interaction with Na(V)1.7. Disruption of this interaction by a membrane-permeable competitive peptide, GST-Flag-Na(V)1.7CT-TAT, reduced the percentage of histamine-responsive DRG neurons, and impaired the histamine-induced scratching, indicating that the FGF13/Na(V)1.7 interaction is a key molecular determinant in the histamine-induced itch sensation. Therefore, our study reveals a novel role of FGF13 in mediating itch sensation via the interaction of Na(V)1.7 in the peripheral nervous system.
关键词	DRG fibroblast growth factor 13 histamine itch sensation sodium channel
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31700901][31671058] ; Strategic Priority Research Program (B) of Chinese Academy of Sciences[XDB39000000] ; Key Research Program of Frontier Sciences[QYZDY-SSW-SMC007] ; Chinese Academy of Sciences, Science and Technology Commission of Shanghai Municipality, China[18JC1420301] ; CAMS Innovation Fund for Medical Sciences[2019-I2M-5-082]
WOS研究方向	Neurosciences & Neurology
WOS类目	Neurosciences
WOS记录号	WOS:000606706400006
出版者	SOC NEUROSCIENCE
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125035
专题	生命科学与技术学院_特聘教授组_张旭组生命科学与技术学院_特聘教授组_鲍岚组生命科学与技术学院_博士生
通讯作者	Bao, Lan; Zhang, Xu
作者单位	1.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, State Key Lab Neurosci, Shanghai 200031, Peoples R China 3.Chinese Acad Sci, State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Inst Brain Intelligence Sci & Technol, Zhangjiang Lab, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Shanghai Ctr Brain Sci & BrainInspired Intelligen, Res Unit Pain, Shanghai 201210, Peoples R China 6.Chinese Acad Sci, Xu Hui Cent Hosp, Shanghai Clin Res Ctr, Shanghai 200031, Peoples R China 7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Dong, Fei,Shi, Haixiang,Yang, Liu,et al. FGF13 Is Required for Histamine-Induced Itch Sensation by Interaction with Na(V)1.7[J]. JOURNAL OF NEUROSCIENCE,2020,40(50):9589-9601.
APA	Dong, Fei.,Shi, Haixiang.,Yang, Liu.,Xue, Huaqing.,Wei, Manyi.,...&Zhang, Xu.(2020).FGF13 Is Required for Histamine-Induced Itch Sensation by Interaction with Na(V)1.7.JOURNAL OF NEUROSCIENCE,40(50),9589-9601.
MLA	Dong, Fei,et al."FGF13 Is Required for Histamine-Induced Itch Sensation by Interaction with Na(V)1.7".JOURNAL OF NEUROSCIENCE 40.50(2020):9589-9601.