AtMYB109 negatively regulates stomatal closure under osmotic stress in Arabidopsis thaliana

doi:10.1016/j.jplph.2020.153292

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	AtMYB109 negatively regulates stomatal closure under osmotic stress in Arabidopsis thaliana
	So, Won Mi 1; Huque, A. K. M. Mahmudul 1; Shin, Hyun-young 1; Kim, Soo Youn 1,2; Shin, Jin Seok 1; Cui, Meihua1,3 ; Shin, Jeong Sheop 1
	2020-12
发表期刊	JOURNAL OF PLANT PHYSIOLOGY (IF:4.0[JCR-2023],4.1[5-Year])
ISSN	0176-1617
卷号	255
发表状态	已发表
DOI	10.1016/j.jplph.2020.153292
摘要	Osmotic stress, caused by drought, salinity, or PEG (polyethylene glycol), is one of the most important abiotic factors that hinder plant growth and development. In Arabidopsis, more than 100 R2R3-MYB transcription factors (TFs) have been identified, and many of them are involved in the transcriptional regulation of a variety of biological processes related to growth and development, as well as responses to biotic and abiotic stresses. However, the MYB TF involving in both plant development and stress response has rarely been reported. We report here that Arabidopsis AtMYB109, a R2R3-MYB TF, functions as a negative regulator of stomatal closure under osmotic stress as well as of pollen tube elongation. Under PEG-induced osmotic stress, whole leaves of AtMYB109-OXs were intensely wilted, while leaves of the wild-type (WT) and myb109 were weakly affected. Moreover, we confirmed that the wilting in AtMYB109-OXs was more severe than in WT and myb109 under drought conditions, and that after re-watering, WT and myb109 plants promptly recovered, while AtMYB109-OXs failed to survive. In addition, stomatal closure was delayed in the AtMYB109-OXs compared to the WT and myb109. However, proline content and the expression of stress-induced and proline synthesis genes were higher in the overexpression lines than in WT and myb109. Then, we observed that the expression of ICS1, a key gene in SA biosynthesis, was greatly suppressed in AtMYB109-OXs. In addition, we found that AtMYB109 expression gradually increased until the flowers were fully opened and thereafter dramatically decreased during silique development. The pollen tube growth was significantly suppressed in AtMYB109-OXs compared to the WT and myb109. Using EMSA and ChIP-qPCR, we confirmed that AtMYB109 bound to the promoter of RABA4D, a gene encoding a pollen development regulator. Taken together, we suggest the delayed stomatal closing and vulnerable phenotypes in the AtMYB109-OXs under osmotic stress are possibly directly or indirectly associated with a SA-mediated mechanism, and that AtMYB109 suppresses RABA4D that modulates pollen tube growth.
关键词	Arabidopsis AtMYB109 Osmotic stress Pollen tube growth
收录类别	SCI ; SCIE
语种	英语
资助项目	Next-Generation BioGreen 21 Program - Rural Development Administration[PJ011065][PJ01367001] ; National Research Foundation of Korea (NRF) - Ministry of Science, Republic of Korea[2019R1F1A1060014]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000589897400004
出版者	ELSEVIER GMBH
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124325
专题	免疫化学研究所_特聘教授组_生物工程学实验室免疫化学研究所_特聘教授组_干细胞生物学实验室
通讯作者	Shin, Jeong Sheop
作者单位	1.Korea Univ, Div Life Sci, Seoul 02841, South Korea 2.Bionics, Cloning Dept, Seoul 04778, South Korea 3.Shanghai Tech Univ, Shanghai Inst Adv Immunochem Studies SIAIS, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	So, Won Mi,Huque, A. K. M. Mahmudul,Shin, Hyun-young,et al. AtMYB109 negatively regulates stomatal closure under osmotic stress in Arabidopsis thaliana[J]. JOURNAL OF PLANT PHYSIOLOGY,2020,255.
APA	So, Won Mi.,Huque, A. K. M. Mahmudul.,Shin, Hyun-young.,Kim, Soo Youn.,Shin, Jin Seok.,...&Shin, Jeong Sheop.(2020).AtMYB109 negatively regulates stomatal closure under osmotic stress in Arabidopsis thaliana.JOURNAL OF PLANT PHYSIOLOGY,255.
MLA	So, Won Mi,et al."AtMYB109 negatively regulates stomatal closure under osmotic stress in Arabidopsis thaliana".JOURNAL OF PLANT PHYSIOLOGY 255(2020).