Mediator Med23 Regulates Adult Hippocampal Neurogenesis

doi:10.3389/fcell.2020.00699

	Mediator Med23 Regulates Adult Hippocampal Neurogenesis
	Chen, Guo-Yan 1,2,3; Zhang, Shuai4,5 ; Li, Chong-Hui 4; Qi, Cong-Cong 6,7,8; Wang, Ya-Zhou 9; Chen, Jia-Yin 2,3; Wang, Gang 4,10; Ding, Yu-Qiang 2,3,6,7,8; Su, Chang-Jun 1
	2020-07-29
发表期刊	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (IF:4.6[JCR-2023],5.2[5-Year])
ISSN	2296-634X
卷号	8
发表状态	已发表
DOI	10.3389/fcell.2020.00699
摘要	Mammalian Mediator (Med) is a key regulator of gene expression by linking transcription factors to RNA polymerase II (Pol II) transcription machineries. The Mediator subunit 23 (Med23) is a member of the conserved Med protein complex and plays essential roles in diverse biological processes including adipogenesis, carcinogenesis, osteoblast differentiation, and T-cell activation. However, its potential functions in the nervous system remain unknown. We report here that Med23 is required for adult hippocampal neurogenesis in mouse. Deletion of Med23 in adult hippocampal neural stem cells (NSCs) was achieved in Nestin-Cre(ER):Med23(flox/flox)mice by oral administration of tamoxifen. We found an increased number of proliferating NSCs shown by pulse BrdU-labeling and immunostaining of MCM2 and Ki67, which is possibly due to a reduction in cell cycle length, with unchanged GFAP(+)/Sox2(+)NSCs and Tbr2(+)progenitors. On the other hand, neuroblasts and immature neurons indicated by NeuroD and DCX were decreased in number in the dentate gyrus (DG) of Med23-deficient mice. In addition, these mice also displayed defective dendritic morphogenesis, as well as a deficiency in spatial and contextual fear memory. Gene ontology (GO) analysis of hippocampal NSCs revealed an enrichment in genes involved in cell proliferation, Pol II-associated transcription, Notch signaling pathway and apoptosis. These results demonstrate that Med23 plays roles in regulating adult brain neurogenesis and functions.
关键词	Mediator complex 23 hippocampus proliferation cell cycle adult neural stem cells
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收录类别	SCI ; SCIE
语种	英语
资助项目	Shanghai Municipal Science and Technology Major Project[2018SHZDZX01][19490714300] ; National Natural Science Foundation of China[31671543][81571332][91232724][31371094][81701327] ; National Key R&D Program of China[2017YFA0104002][2017YFA0102700]
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000561492600001
出版者	FRONTIERS MEDIA SA
WOS关键词	IMMEDIATE-EARLY GENES ; CELL-CYCLE LENGTH ; INTELLECTUAL DISABILITY ; SYNAPTIC PLASTICITY ; COACTIVATOR COMPLEX ; CENTRAL SEROTONIN ; PRECURSOR CELLS ; STEM-CELLS ; S-PHASE ; TRANSCRIPTION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123067
专题	生命科学与技术学院_博士生
通讯作者	Wang, Gang; Ding, Yu-Qiang; Su, Chang-Jun
作者单位	1.Fourth Mil Med Univ, Air Force Med Univ, Tangdu Hosp, Dept Neurol, Xian, Peoples R China 2.Tongji Univ, Sch Med, East Hosp, Key Lab Arrhythmias,Minist Educ China, Shanghai, Peoples R China 3.Tongji Univ, Sch Med, Dept Anat & Neurobiol, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Univ Chinese Acad Sci, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai, Peoples R China 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 6.Fudan Univ, State Key Lab Med Neurobiol, Shanghai, Peoples R China 7.Fudan Univ, MOE Frontiers Ctr Brain Sci, Inst Brain Sci, Shanghai, Peoples R China 8.Fudan Univ, Dept Lab Anim Sci, Shanghai, Peoples R China 9.Fourth Mil Med Univ, Sch Basic Med, Inst Neurosci, Dept Neurobiol, Xian, Peoples R China 10.Fudan Univ, Sch Life Sci, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guo-Yan,Zhang, Shuai,Li, Chong-Hui,et al. Mediator Med23 Regulates Adult Hippocampal Neurogenesis[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2020,8.
APA	Chen, Guo-Yan.,Zhang, Shuai.,Li, Chong-Hui.,Qi, Cong-Cong.,Wang, Ya-Zhou.,...&Su, Chang-Jun.(2020).Mediator Med23 Regulates Adult Hippocampal Neurogenesis.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,8.
MLA	Chen, Guo-Yan,et al."Mediator Med23 Regulates Adult Hippocampal Neurogenesis".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 8(2020).