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Segmental Expression of the Bradykinin Type 2 Receptor in Rat Efferent Ducts and Epididymis and Its Role in the Regulation of Aquaporin 9 | |
2009 | |
发表期刊 | BIOLOGY OF REPRODUCTION |
ISSN | 0006-3363 |
EISSN | 1529-7268 |
卷号 | 80期号:1页码:134-143 |
发表状态 | 已发表 |
DOI | 10.1095/biolreprod.108.070797 |
摘要 | Water and solute transport in the efferent ducts and epididymis are important for the establishment of the appropriate luminal environment for sperm maturation and storage. Aquaporin 9 (AQP9) is the main water channel in the epididymis, but its regulation is still poorly understood. Components of the kinin-kallikrein system (KKS), leading to the production of bradykinin (BK), are highly expressed in the lumen of the male reproductive tract. We report here that the epididymal luminal fluid contains a significant amount of BK (2 nM). RT-PCR performed on epididymal epithelial cells isolated by laser capture microdissection (LCM) showed abundant BK type 2 receptor (Bdkrb2) mRNA expression but no type 1 receptor (Bdkrb1). Double-immunofluorescence staining for BDKRB2 and the anion exchanger AE2 (a marker of efferent duct ciliated cells) or the V-ATPase E subunit, official symbol ATP6V1E1 (a marker of epididymal clear cells), showed that BDKRB2 is expressed in the apical pole of nonciliated cells (efferent ducts) and principal cells (epididymis). Triple labeling for BDKRB2, AQP9, and ATP6V1E1 showed that BDKRB2 and AQP9 colocalize in the apical stereocilia of principal cells in the cauda epididymidis. While uniform Bdkrb2 mRNA expression was detected in the efferent ducts and along the epididymal tubule, marked variations were detected at the protein level. BDKRB2 was highest in the efferent ducts and cauda epididymidis, intermediate in the distal initial segment, moderate in the corpus, and undetectable in the proximal initial segment and the caput. Functional assays on tubules isolated from the distal initial segments showed that BK significantly increased AQP9-dependent glycerol apical membrane permeability. This effect was inhibited by BAPTA-AM, demonstrating the participation of calcium in this process. This study, therefore, identifies BK as an important regulator of AQP9. |
关键词 | bradykinin receptor efferent ducts epididymis male reproductive tract water channel |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Reproductive Biology |
WOS类目 | Reproductive Biology |
WOS记录号 | WOS:000262010100015 |
WOS关键词 | MALE REPRODUCTIVE-TRACT ; TRANSMEMBRANE CONDUCTANCE REGULATOR ; ANGIOTENSIN-CONVERTING ENZYME ; KALLIKREIN-KININ SYSTEM ; V-ATPASE ; MALE-FERTILITY ; VAS-DEFERENS ; ANION SECRETION ; BLOOD-PRESSURE ; B-2 RECEPTOR |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121685 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Breton, S. |
作者单位 | 1.Massachusetts Gen Hosp, Program Membrane Biol, Ctr Syst Biol, Div Nephrol, Boston, MA 02114 USA 2.Univ Montreal, Grp Etud Prot Membranaires, Montreal, PQ H3C 3J7, Canada |
推荐引用方式 GB/T 7714 | Belleannee, C.,Da Silva, N.,Shum, W. W. C.,et al. Segmental Expression of the Bradykinin Type 2 Receptor in Rat Efferent Ducts and Epididymis and Its Role in the Regulation of Aquaporin 9[J]. BIOLOGY OF REPRODUCTION,2009,80(1):134-143. |
APA | Belleannee, C..,Da Silva, N..,Shum, W. W. C..,Marsolais, M..,Laprade, R..,...&Breton, S..(2009).Segmental Expression of the Bradykinin Type 2 Receptor in Rat Efferent Ducts and Epididymis and Its Role in the Regulation of Aquaporin 9.BIOLOGY OF REPRODUCTION,80(1),134-143. |
MLA | Belleannee, C.,et al."Segmental Expression of the Bradykinin Type 2 Receptor in Rat Efferent Ducts and Epididymis and Its Role in the Regulation of Aquaporin 9".BIOLOGY OF REPRODUCTION 80.1(2009):134-143. |
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