CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions

doi:10.1016/j.str.2020.04.010

	CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions
	Ochi, Takashi 1,14; Quarantotti, Valentina 2; Lin, Huawen 3; Jullien, Jerome 4,5,6; Rosa e Silva, Ivan 1; Boselli, Francesco 7; Barnabas, Deepak D.1; Johnson, Christopher M.1; McLaughlin, Stephen H.1; Freund, Stefan M., V 1; Blackford, Andrew N.8,9; Kimata, Yuu10,11 ; Goldstein, Raymond E.7; Jackson, Stephen P.4,12; Blundell, Tom L.12; Dutcher, Susan K.3; Gergely, Fanni 2; van Breugel, Mark 1,13
	2020-06-02
发表期刊	STRUCTURE (IF:4.4[JCR-2023],4.3[5-Year])
ISSN	0969-2126
EISSN	1878-4186
卷号	28 期号:6 页码:674-+
发表状态	已发表
DOI	10.1016/j.str.2020.04.010
摘要	Centrioles are cylindrical assemblies whose peripheral microtubule array displays a 9-fold rotational symmetry that is established by the scaffolding protein SAS6. Centriole symmetry can be broken by centriole-associated structures, such as the striated fibers in Chlamydomonas that are important for ciliary function. The conserved protein CCDC61/VFL3 is involved in this process, but its exact role is unclear Here, we show that CCDC61 is a paralog of SAS6. Crystal structures of CCDC61 demonstrate that it contains two homodimerization interfaces that are similar to those found in SAS6, but result in the formation of linear filaments rather than rings. Furthermore, we show that CCDC61 binds microtubules and that residues involved in CCDC61 microtubule binding are important for ciliary function in Chlamydomonas. Together, our findings suggest that CCDC61 and SAS6 functionally diverged from a common ancestor while retaining the ability to scaffold the assembly of basal body-associated structures or centrioles, respectively.
收录类别	SCI ; SCIE
语种	英语
资助项目	Medical Research Council (MRC)[MC_UP_1201/3] ; Cancer Research UK[C14303/A17043][C6/A18796][CRUK-A12874] ; National Institute of General Medical Sciences[GM-032843][GM-131909] ; Wellcome Trust[09316/Z/10/Z][200814/Z/16/Z][206388/Z/17/Z][101050/Z/13/Z][207510/Z/17/Z] ; Cancer Research UK Career Development Fellowship[C29215/A20772] ; MRC[MR/P00479/1]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号	WOS:000538119800011
出版者	CELL PRESS
WOS关键词	STRUCTURE PREDICTION ; SEQUENCE ALIGNMENT ; CRYSTAL-STRUCTURE ; BASAL BODIES ; CHLAMYDOMONAS ; CENTRIOLE ; CENTROSOME ; PROTEINS ; XRCC4 ; COMPONENT
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121679
专题	生命科学与技术学院_PI研究组_Yuu Kimita组
通讯作者	Ochi, Takashi; van Breugel, Mark
作者单位	1.MRC Lab Mol Biol, Cambridge Biomed Campus,Francis Crick Ave, Cambridge CB2 0QH, England 2.Univ Cambridge, Li Ka Shing Ctr, Canc Res UK Cambridge Inst, Robinson Way, Cambridge CB2 0RE, England 3.Washington Univ, Dept Genet, Sch Med, 452 Clayton Ave, St Louis, MO 63110 USA 4.Univ Cambridge, Wellcome Trust Canc Res UK Gurdon Inst, Cambridge CB2 1QN, England 5.Univ Cambridge, Dept Zool, Downing St, Cambridge CB2 3EJ, England 6.Univ Nantes, INSERM, CRTI, Nantes, France 7.Ctr Math Sci, DAMTP, Wilberforce Rd, Cambridge CB3 0WA, England 8.Univ Oxford, John Radcliffe Hosp, MRC Weatherall Inst Mol Med, Dept Oncol, Oxford OX3 9DS, England 9.Univ Oxford, Oxford Inst Radiat Oncol, Canc Res UK & Med Res Council, Oxford OX3 7DQ, England 10.Univ Cambridge, Dept Genet, Cambridge CB4 1AR, England 11.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 12.Univ Cambridge, Dept Biochem, 80 Tennis Court Rd, Cambridge CB2 1GA, England 13.Queen Mary Univ London, Sch Biol & Chem Sci, Abernethy Bldg,Room 3-19,2 Newark St, London E1 2AT, England 14.Univ Leeds, Fac Biol Sci, Astbury Ctr Struct Mol Biol, Sch Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England
推荐引用方式 GB/T 7714	Ochi, Takashi,Quarantotti, Valentina,Lin, Huawen,et al. CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions[J]. STRUCTURE,2020,28(6):674-+.
APA	Ochi, Takashi.,Quarantotti, Valentina.,Lin, Huawen.,Jullien, Jerome.,Rosa e Silva, Ivan.,...&van Breugel, Mark.(2020).CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.STRUCTURE,28(6),674-+.
MLA	Ochi, Takashi,et al."CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions".STRUCTURE 28.6(2020):674-+.