Conformable self-assembling amyloid protein coatings with genetically programmable functionality

doi:10.1126/sciadv.aba1425

	Conformable self-assembling amyloid protein coatings with genetically programmable functionality
	Li, Yingfeng1 ; Li, Ke1 ; Wang, Xinyu1 ; Cui, Mengkui1 ; Ge, Peng 2; Zhang, Junhu 2; Qiu, Feng3 ; Zhong, Chao1,4
	2020-05
发表期刊	SCIENCE ADVANCES
ISSN	2375-2548
卷号	6 期号:21
发表状态	已发表
DOI	10.1126/sciadv.aba1425
摘要	Functional coating materials have found broad technological applications in diverse fields. Despite recent advances, few coating materials simultaneously achieve robustness and substrate independence while still retaining the capacity for genetically encodable functionalities. Here, we report Escherichia coli biofilm-inspired protein nanofiber coatings that simultaneously exhibit substrate independence, resistance to organic solvents, and programmable functionalities. The intrinsic surface adherence of CsgA amyloid proteins, along with a benign solution-based fabrication approach, facilitates forming nanofiber coatings on virtually any surface with varied compositions, sizes, shapes, and structures. In addition, the typical amyloid structures endow the nanofiber coatings with outstanding robustness. On the basis of their genetically engineerable functionality, our nanofiber coatings can also seamlessly participate in functionalization processes, including gold enhancement, diverse protein conjugations, and DNA binding, thus enabling a variety of proof-of-concept applications, including electronic devices, enzyme immobilization, and microfluidic bacterial sensors. We envision that our coatings can drive advances in electronics, biocatalysis, particle engineering, and biomedicine.
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收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	Commission for Science and Technology of Shanghai Municipality[17JC1403900] ; National Natural Science Foundation of China[U1932204] ; National Science and Technology Major Project of the Ministry of Science and Technology of China[2018YFA0902804]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000537235300035
出版者	AMER ASSOC ADVANCEMENT SCIENCE
WOS关键词	SURFACE MODIFICATION ; PEPTIDE ; NANOFILM ; BINDING ; FILM
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121630
专题	物质科学与技术学院_博士生物质科学与技术学院_PI研究组_钟超组生命科学与技术学院_本科生
通讯作者	Zhong, Chao
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Mat & Phys Biol Div, Shanghai 201210, Peoples R China 2.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 4.Chinese Acad Sci, Mat Synthet Biol Ctr, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
第一作者单位	物质科学与技术学院
通讯作者单位	物质科学与技术学院
第一作者的第一单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Li, Yingfeng,Li, Ke,Wang, Xinyu,et al. Conformable self-assembling amyloid protein coatings with genetically programmable functionality[J]. SCIENCE ADVANCES,2020,6(21).
APA	Li, Yingfeng.,Li, Ke.,Wang, Xinyu.,Cui, Mengkui.,Ge, Peng.,...&Zhong, Chao.(2020).Conformable self-assembling amyloid protein coatings with genetically programmable functionality.SCIENCE ADVANCES,6(21).
MLA	Li, Yingfeng,et al."Conformable self-assembling amyloid protein coatings with genetically programmable functionality".SCIENCE ADVANCES 6.21(2020).