ShanghaiTech University Knowledge Management System
| Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale | |
| 2020-05-06 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 卷号 | 15期号:5 |
| 发表状态 | 已发表 |
| DOI | 10.1371/journal.pone.0232081 |
| 摘要 | The reproduction of reliable in vitro models of human skeletal muscle is made harder by the intrinsic 3D structural complexity of this tissue. Here we coupled engineered hydrogel with 3D structural cues and specific mechanical properties to derive human 3D muscle constructs ("myobundles") at the scale of single fibers, by using primary myoblasts or myoblasts derived from embryonic stem cells. To this aim, cell culture was performed in confined, laminin-coated micrometric channels obtained inside a 3D hydrogel characterized by the optimal stiffness for skeletal muscle myogenesis. Primary myoblasts cultured in our 3D culture system were able to undergo myotube differentiation and maturation, as demonstrated by the proper expression and localization of key components of the sarcomere and sarcolemma. Such approach allowed the generation of human myobundles of similar to 10 mm in length and similar to 120 mu m in diameter, showing spontaneous contraction 7 days after cell seeding. Transcriptome analyses showed higher similarity between 3D myobundles and skeletal signature, compared to that found between 2D myotubes and skeletal muscle, mainly resulting from expression in 3D myobundles of categories of genes involved in skeletal muscle maturation, including extracellular matrix organization. Moreover, imaging analyses confirmed that structured 3D culture system was conducive to differentiation/maturation also when using myoblasts derived from embryonic stem cells. In conclusion, our structured 3D model is a promising tool for modelling human skeletal muscle in healthy and diseases conditions. |
| URL | 查看原文 |
| 收录类别 | SCI ; SCIE |
| 语种 | 英语 |
| 资助项目 | Oak Foundation[W1095/OCAY-14-191] ; STARS Starting Grant 2017[LS3-19613] |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000537283500019 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS关键词 | MYOGENESIS ; INJURY ; REPAIR ; BIOMATERIALS ; HYDROGELS ; LAMININ ; VIVO |
| 原始文献类型 | Article |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121628 |
| 专题 | 免疫化学研究所_特聘教授组_生物工程学实验室 |
| 通讯作者 | Vitiello, Libero; Elvassore, Nicola |
| 作者单位 | 1.Univ Padua, Ind Engn Dept, Padua, Italy 2.Venetian Inst Mol Med, Padua, Italy 3.Univ Padua, Womens & Childrens Hlth Dept, Padua, Italy 4.Univ Padua, Dept Biol, Padua, Italy 5.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China 6.Univ Verona, Dept Biotechnol, Verona, Italy 7.Interuniv Inst Myol IIM, Assisi, Italy 8.Univ Coll London ICH, London, England |
| 通讯作者单位 | 免疫化学研究所 |
| 推荐引用方式 GB/T 7714 | Urciuolo, Anna,Serena, Elena,Ghua, Rusha,et al. Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale[J]. PLOS ONE,2020,15(5). |
| APA | Urciuolo, Anna.,Serena, Elena.,Ghua, Rusha.,Zatti, Susi.,Giomo, Monica.,...&Elvassore, Nicola.(2020).Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale.PLOS ONE,15(5). |
| MLA | Urciuolo, Anna,et al."Engineering a 3D in vitro model of human skeletal muscle at the single fiber scale".PLOS ONE 15.5(2020). |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
修改评论
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。