A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry

doi:10.1016/j.chroma.2020.461215

	A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry
	Zhang, Hanzhi 1,2; Lou, Chunli1,3 ; Li, Jing1,3 ; Kang, Jingwu1,3
	2020-08-02
发表期刊	JOURNAL OF CHROMATOGRAPHY A (IF:3.8[JCR-2023],3.5[5-Year])
ISSN	18733778
卷号	1624
发表状态	已发表
DOI	10.1016/j.chroma.2020.461215
摘要	A method for the preparation of an on-column ESI emitter used as the sheathless interface for coupling capillary electrophoresis (CE) with mass spectrometry (MS) was developed. The emitter was directly fabricated at the outlet end of the separation capillary which was etched with HF solution to a symmetrical tip. The tip was covered with a small piece of gold foil which was fixed by epoxy resin glue for electrical contact. Such a prepared ESI emitter can produce a stable ESI signal over the wide range of flow rate from 50 nL/min to 800 nL/min. The performance of the CE-MS with the sheathless interface was evaluated by using the separation of four alkaloids. It was found that the strong electroosmotic flow produced by the multiple polyelectrolyte coating on the capillary is necessary for maintaining a stable MS signal. Effect of the running buffer composition, concentration and the CE separation voltages on the ESI signal strength were investigated. The absolute detection limits for the alkaloids was determined as fmol level. Moreover, the CE-MS was applied for the analyses of trypsin digestion of cytochrome C and small molecular organic anions. The emitter performed very stable with a lifetime of at least 180 h. © 2020 Elsevier B.V.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundations of China[21775158][21375140][21175146] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB20020200]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
出版者	Elsevier B.V.
EI入藏号	20202108691629
EI主题词	Alkaloids ; Capillary electrophoresis ; Electroosmosis ; Epoxy resins ; Gold ; Hydrofluoric acid ; Mass spectrometry ; Polyelectrolytes
EI分类号	Precious Metals:547.1 ; Electricity: Basic Concepts and Phenomena:701.1 ; Chemistry:801 ; Chemical Products Generally:804 ; Inorganic Compounds:804.2 ; Glass:812.3 ; Organic Polymers:815.1.1
WOS关键词	COATED EMITTER ; PERFORMANCE ; SEPARATION ; DESIGN
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121575
专题	物质科学与技术学院_硕士生物质科学与技术学院_特聘教授组_康经武组物质科学与技术学院_博士生
通讯作者	Kang, Jingwu
作者单位	1.State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai; 200032, China 2.Shanghai Institute for Food and Drug Control, Zhangheng Road 1500, Shanghai; 201203, China 3.School of Physical Science and Technology, ShanghaiTech University, Haike Road 100, Shanghai; 200120, China
通讯作者单位	物质科学与技术学院
推荐引用方式 GB/T 7714	Zhang, Hanzhi,Lou, Chunli,Li, Jing,et al. A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1624.
APA	Zhang, Hanzhi,Lou, Chunli,Li, Jing,&Kang, Jingwu.(2020).A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1624.
MLA	Zhang, Hanzhi,et al."A gold foil covered fused silica capillary tip as a sheathless interface for coupling capillary electrophoresis-mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1624(2020).