Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae

doi:10.1186/s12934-020-01347-8

	Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae
	Zhang, Zhongxi 1,2,5; Yang, Yang 1,2,5; Wang, Yike 1,2,5; Gu, Jinjie 2,5; Lu, Xiyang 2; Liao, Xianyan 1; Shi, Jiping2,6 ; Kim, Chul Ho 4; Lye, Gary 3; Baganz, Frank 3; Hao, Jian 2,3
	2020-04-15
发表期刊	MICROBIAL CELL FACTORIES (IF:4.3[JCR-2023],5.5[5-Year])
EISSN	1475-2859
卷号	19 期号:1
发表状态	已发表
DOI	10.1186/s12934-020-01347-8
摘要	Background Biological routes for ethylene glycol production have been developed in recent years by constructing the synthesis pathways in different microorganisms. However, no microorganisms have been reported yet to produce ethylene glycol naturally. Results Xylonic acid utilizing microorganisms were screened from natural environments, and an Enterobacter cloacae strain was isolated. The major metabolites of this strain were ethylene glycol and glycolic acid. However, the metabolites were switched to 2,3-butanediol, acetoin or acetic acid when this strain was cultured with other carbon sources. The metabolic pathway of ethylene glycol synthesis from xylonic acid in this bacterium was identified. Xylonic acid was converted to 2-dehydro-3-deoxy-d-pentonate catalyzed by d-xylonic acid dehydratase. 2-Dehydro-3-deoxy-d-pentonate was converted to form pyruvate and glycolaldehyde, and this reaction was catalyzed by an aldolase. d-Xylonic acid dehydratase and 2-dehydro-3-deoxy-d-pentonate aldolase were encoded by yjhG and yjhH, respectively. The two genes are part of the same operon and are located adjacent on the chromosome. Besides yjhG and yjhH, this operon contains four other genes. However, individually inactivation of these four genes had no effect on either ethylene glycol or glycolic acid production; both formed from glycolaldehyde. YqhD exhibits ethylene glycol dehydrogenase activity in vitro. However, a low level of ethylene glycol was still synthesized by E. cloacae Delta yqhD. Fermentation parameters for ethylene glycol and glycolic acid production by the E. cloacae strain were optimized, and aerobic cultivation at neutral pH were found to be optimal. In fed batch culture, 34 g/L of ethylene glycol and 13 g/L of glycolic acid were produced in 46 h, with a total conversion ratio of 0.99 mol/mol xylonic acid. Conclusions A novel route of xylose biorefinery via xylonic acid as an intermediate has been established.
关键词	Enterobacter cloacae Ethylene glycol Glycolic acid Xylonic acid Xylose
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Key R&D Program of China[2017YFE0112700] ; NAF[\R2\180721] ; Natural Science Foundation of Shanghai[19ZR1463600] ; National Natural Science Foundation of China[21576279]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000537126300001
出版者	BMC
WOS关键词	HIGH-YIELD PRODUCTION ; GENE REPLACEMENT ; ESCHERICHIA-COLI ; LAMBDA RED ; INACTIVATION ; PATHWAY
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121552
专题	生命科学与技术学院_特聘教授组_史吉平组
通讯作者	Baganz, Frank; Hao, Jian
作者单位	1.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery, Shanghai 201210, Peoples R China 3.UCL, Dept Biochem Engn, Gordon St, London WC1H 0AH, England 4.KRIBB, Microbial Biotechnol Res Ctr, Jeonbuk Branch Inst, Jeongeup 556212, Jeonbuk, South Korea 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhongxi,Yang, Yang,Wang, Yike,et al. Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae[J]. MICROBIAL CELL FACTORIES,2020,19(1).
APA	Zhang, Zhongxi.,Yang, Yang.,Wang, Yike.,Gu, Jinjie.,Lu, Xiyang.,...&Hao, Jian.(2020).Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae.MICROBIAL CELL FACTORIES,19(1).
MLA	Zhang, Zhongxi,et al."Ethylene glycol and glycolic acid production from xylonic acid by Enterobacter cloacae".MICROBIAL CELL FACTORIES 19.1(2020).