The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe

doi:10.3390/cells9040955

KMS > 生命科学与技术学院 > PI研究组 > Tomoyasu Sugiyama组

	The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe
	Marayati, Bahjat F.1; Tucker, James F.1; De La Cerda, David A.1; Hou, Tien-Chi 1; Chen, Rong 2; Sugiyama, Tomoyasu3 ; Pease, James B.1; Zhang, Ke 1
	2020-04
发表期刊	CELLS
EISSN	2073-4409
卷号	9 期号:4
发表状态	已发表
DOI	10.3390/cells9040955
摘要	In eukaryotes, heterochromatin plays a critical role in organismal development and cell fate acquisition, through regulating gene expression. The evolutionarily conserved lysine-specific demethylases, Lsd1 and Lsd2, remove mono- and dimethylation on histone H3, serving complex roles in gene expression. In the fission yeast Schizosaccharomyces pombe, null mutations of Lsd1 and Lsd2 result in either severe growth defects or inviability, while catalytic inactivation causes minimal defects, indicating that Lsd1 and Lsd2 have essential functions beyond their known demethylase activity. Here, we show that catalytic mutants of Lsd1 or Lsd2 partially assemble functional heterochromatin at centromeres in RNAi-deficient cells, while the C-terminal truncated alleles of Lsd1 or Lsd2 exacerbate heterochromatin formation at all major heterochromatic regions, suggesting that Lsd1 and Lsd2 repress heterochromatic transcripts through mechanisms both dependent on and independent of their catalytic activities. Lsd1 and Lsd2 are also involved in the establishment and maintenance of heterochromatin. At constitutive heterochromatic regions, Lsd1 and Lsd2 regulate one another and cooperate with other histone modifiers, including the class II HDAC Clr3 and the Sirtuin family protein Sir2 for gene silencing, but not with the class I HDAC Clr6. Our findings explore the roles of lysine-specific demethylases in epigenetic gene silencing at heterochromatic regions.
关键词	Lsd1 Lsd2 heterochromatin lysine demethylase gene silencing H3K9me2
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	NIH[1R15GM119105 - 01]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000535559500166
出版者	MDPI
WOS关键词	JMJC DOMAIN PROTEIN ; HISTONE H3 METHYLATION ; FISSION YEAST ; RNA INTERFERENCE ; EPIGENETIC INHERITANCE ; GENE ACTIVATION ; TRANSCRIPTION ; NUCLEATION ; COMPLEX ; CHROMATIN
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121527
专题	生命科学与技术学院_PI研究组_Tomoyasu Sugiyama组
通讯作者	Zhang, Ke
作者单位	1.Wake Forest Univ, Dept Biol, Ctr Mol Signaling, Winston Salem, NC 27109 USA 2.Wake Forest Sch Med, Physiol & Pharmacol, Winston Salem, NC 27101 USA 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Marayati, Bahjat F.,Tucker, James F.,De La Cerda, David A.,et al. The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe[J]. CELLS,2020,9(4).
APA	Marayati, Bahjat F..,Tucker, James F..,De La Cerda, David A..,Hou, Tien-Chi.,Chen, Rong.,...&Zhang, Ke.(2020).The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe.CELLS,9(4).
MLA	Marayati, Bahjat F.,et al."The Catalytic-Dependent and -Independent Roles of Lsd1 and Lsd2 Lysine Demethylases in Heterochromatin Formation in Schizosaccharomyces pombe".CELLS 9.4(2020).