Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast
Sugiyama, Tomoyasu; Wanatabe, Nobuyoshi; Kitahata, Eri; Tani, Tokio; Sugioka-Sugiyama, Rie
2013
发表期刊NUCLEIC ACIDS RESEARCH
ISSN0305-1048
EISSN1362-4962
卷号41期号:13页码:6674-6686
发表状态已发表
DOI10.1093/nar/gkt363
摘要

Zinc-finger domains are found in many nucleic acid-binding proteins in both prokaryotes and eukaryotes. Proteins carrying zinc-finger domains have important roles in various nuclear transactions, including transcription, mRNA processing and mRNA export; however, for many individual zinc-finger proteins in eukaryotes, the exact function of the protein is not fully understood. Here, we report that Red5 is involved in efficient suppression of specific mRNAs during vegetative growth of Schizosaccharomyces pombe. Red5, which contains five C3H1-type zinc-finger domains, localizes to the nucleus where it forms discrete dots. A red5 point mutation, red5-2, results in the upregulation of specific meiotic mRNAs in vegetative mutant red5-2 cells; northern blot data indicated that these meiotic mRNAs in red5-2 cells have elongated poly(A) tails. RNA-fluorescence in situ hybridization results demonstrate that poly(A)(+) RNA species accumulate in the nucleolar regions of red5-deficient cells. Moreover, Red5 genetically interacts with several mRNA export factors. Unexpectedly, three components of the nuclear pore complex also suppress a specific set of meiotic mRNAs. These results indicate that Red5 function is important to meiotic mRNA degradation; they also suggest possible connections among selective mRNA decay, mRNA export and the nuclear pore complex in vegetative fission yeast.

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收录类别SCI
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000322458800033
WOS关键词SELECTIVE ELIMINATION ; FUNCTIONALLY INTERACTS ; QUALITY-CONTROL ; PROTEIN ; MEIOSIS ; COMPLEX ; EXPORT ; HOMOLOG ; RAE1P ; POLYADENYLATION
原始文献类型Article
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文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120935
专题个人在本单位外知识产出
通讯作者Sugiyama, Tomoyasu
作者单位1.Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki 3058577, Japan
2.Kumamoto Univ, Fac Sci, Dept Biol Sci, Kumamoto 8608555, Japan
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Sugiyama, Tomoyasu,Wanatabe, Nobuyoshi,Kitahata, Eri,et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast[J]. NUCLEIC ACIDS RESEARCH,2013,41(13):6674-6686.
APA Sugiyama, Tomoyasu,Wanatabe, Nobuyoshi,Kitahata, Eri,Tani, Tokio,&Sugioka-Sugiyama, Rie.(2013).Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast.NUCLEIC ACIDS RESEARCH,41(13),6674-6686.
MLA Sugiyama, Tomoyasu,et al."Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast".NUCLEIC ACIDS RESEARCH 41.13(2013):6674-6686.
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